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Isolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium sp. PCC 6803. | LitMetric

Isolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium sp. PCC 6803.

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Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany. Electronic address:

Published: September 2022

AI Article Synopsis

Article Abstract

The FoF1 ATP synthase (ATPase) is one of the most important protein complexes in energy metabolism. The isolation of functional ATPase complexes is fundamental to address questions about its assembly, regulation, and functions. This protocol describes the purification of intact and active ATPase from the model cyanobacterium sp. PCC 6803. Basis for purification is a 3×FLAG tag fused to the beta subunit. The ATPase is enzymatically active and its purity is demonstrated using mass spectrometry, denaturing, and blue-native PAGE. For complete details on the use and execution of this protocol, please refer to Song et al. (2022).

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418591PMC
http://dx.doi.org/10.1016/j.xpro.2022.101623DOI Listing

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