Renoprotective effects of dapagliflozin in an iron overload non-diabetic rat model.

Purpose: Sodium glucose co-transporter (SGLT) 2 inhibitors are oral anti-diabetic drugs with proven kidney protective effects. Renal protective effects in non-diabetic individuals have also been shown in recent studies. The aim of this study was to determine the renal protective effects of dapagliflozin by evaluating the oxidative stress markers in the kidney tissue and demonstrating it in renal histological sections in an iron-overloaded rat model.

Methods: A total of 24 Wistar Albino rats were separated into 3 groups of 8 rats. Iron sucrose (60 ​mg/kg/day) was administered intraperitoneally to the first group (Group Fe) (n ​= ​8), iron sucrose and dapagliflozin (0.1 ​mg/kg/day) to the second group (Group Fe ​+ ​D) (n ​= ​8) and intraperitoneal saline as placebo to the control group (Group C) (n ​= ​8) for 4 weeks. The glomerular changes were semi-quantitatively scored with Oxford Classification. Oxidative stress was analyzed from the tissue fluorescent oxidation product (FLOP), malondialdehyde (MDA) and total sulfhydryl (T-SH) levels.

Results: Dapagliflozin prevented glomerular and mesangial damage of iron overload in the non-diabetic rat model. MDA levels were significantly higher in Group Fe compared to the Group C, and there was no significant difference between the Fe ​+ ​D group and Group C. T-SH levels were preserved in the Fe ​+ ​D group and were significantly higher than in the Fe group.

Conclusions: The results of this study showed that dapagliflozin helped preserve the glomerular and mesangial structure histologically and reduced oxidative stress markers in a non-diabetic iron overload rat model.