Water contamination is a highly critical issue owing to its strong relationship to human health. In addition to chemical pollutants, microorganisms such as multiresistant pathogenic bacteria have received significant attention from the World Health Organization. The main problem associated with monitoring pathogenic bacteria in water is the interference from concomitant species and their low concentrations. To address this problem, we synthesized a bilanthanide-organic material as an efficient luminescence sensor for the detection of , a representative bacterium, via its two unique biomarkers: 1-hydroxyphenazine (1-HX) and 2-aminoacetophenone (2-AA). This multiplexed sensing approach overcomes a common issue encountered by single-marker luminescence sensors that may report false positives due to coexisting species in the complex environment. High sensitivities and low limits of detection for 1-HX and 2-AA were obtained with very fast response time. The key structural factors governing the high-performance sensing function were revealed. This work provides an alternative route for the effortless and instant detection of bacterial biomarkers in water.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01984 | DOI Listing |
Forensic Sci Int
December 2024
Biological and Environmental Sensing Research Unit, King Abdullah Institute for Nanotechnology, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address:
The forensic Deoxyribonucleic Acid (DNA) fingerprinting is a tool for investigating crime scenes by identifying/tracing criminals and linking crime scenes. However, in cases where experts are unable to detect and identify any biological traces or human-derived cells at the crime scene or while testing the samples in the laboratories, all the advantages offered by forensic laboratories lose their significance. It becomes a waste of time, effort, and resources allocated to these laboratories.
View Article and Find Full Text PDFBiosens Bioelectron
December 2024
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, Jiangsu, People's Republic of China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, 214122, Wuxi, Jiangsu, People's Republic of China; International Joint Laboratory on Food Safety, Jiangnan University, 214122, Wuxi, Jiangsu, People's Republic of China. Electronic address:
Tetracycline antibiotics (TCs) are extensively used as broad-spectrum antimicrobials. However, their excessive use and misuse have led to serious accumulation in foods and environments, posing a significant threat to human health. To solve such public issue, we have designed a novel dual-mode detection method, integrating colorimetric sensing with surface-enhanced Raman scattering (SERS) technology, for sensitive and rapid evaluation on TCs.
View Article and Find Full Text PDFNat Commun
January 2025
Section of Physiology and Biochemistry, Department of Medicine and Surgery, University of Perugia, Perugia, Italy.
Different forms of phosphorylated tau (p-tau) have shown strong potential as Alzheimer's disease (AD) biomarkers in both cerebrospinal fluid (CSF) and plasma. We hypothesized that p-tau proteoforms simultaneously phosphorylated at two different sites may have an increased diagnostic value compared with tau phosphorylated at a single site. Here, we developed two immunoassays detecting CSF and plasma tau simultaneously phosphorylated at both T181 and T231 (p-tau181&231) and at T217 and T231 (p-tau217&231).
View Article and Find Full Text PDFMikrobiyol Bul
October 2024
The University of Groningen, University Medical Center Groningen, Department of Medical Microbiology and Infection Prevention, Division of Clinical Virology, Groningen, Netherlands.
As the number of coronavirus diseases-2019 (COVID-19) cases have decreased and measures have started to be implemented at an individual level rather than in the form of social restrictions, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) still maintains its importance and has already taken its place in the spectrum of agents investigated in multiplex molecular test panels for respiratory tract infections in routine diagnostic use. In this study, we aimed to present mutation analysis and clade distribution of whole genome sequences from randomly selected samples that tested positive with SARS-CoV-2 specific real-time reverse transcription polymerase chain reaction (rRT-PCR) test at different periods of the pandemic in our laboratory with a commercial easy-to-use kit designed for next-generation sequencing systems. A total of 84 nasopharyngeal/oropharyngeal swab samples of COVID-19 suspected patients which were sent for routine diagnosis to the medical microbiology laboratory and detected as SARSCoV-2 RNA positive with rRT-PCR were randomly selected from different periods for sequence analysis.
View Article and Find Full Text PDFBiomed Phys Eng Express
January 2025
Biomechatronics Laboratory, Department of Mechatronics and Mechanical Systems of the Escola Politécnica, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
Characterization of the electroencephalography (EEG) signals related to motor activity, such as alpha- and beta-band motor event-related desynchronizations (ERDs), is essential for Brain Computer Interface (BCI) development. Determining the best electrode combination to detect the ERD is crucial for the success of the BCI. Considering that the EEG signals are bipolar, this involves the choice of the main and reference electrodes.
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