Marker-assisted transfer of leaf and stripe rust resistance from var. cv. Trinakria to wheat variety HD2932.

A marker-assisted backcrossing program initiated to transfer leaf rust resistance gene from cv. Trinakria to hexaploid wheat variety HD2932 cotransferred a stripe rust resistance gene, , along with The cross of hexaploid recurrent parent HD2932 with tetraploid donor parent Trinakria produced pentaploid F plants. Fs were backcrossed with recurrent parent HD2932 to produce BCF generation. Foreground and background selection was conducted in each backcross generation to identify plants for backcrossing or selfing. While foreground selection for was carried out with linked and validated molecular marker , for background selection, 86 polymorphic SSR markers from the A and B genomes were used. Single selected plants from BCF and BCF generations backcrossed and selfed to produce BCFand BCF generations, respectively. Background selection resulted in 83.72%, 91.86%, and 98.25% of RPG recovery in BCF, BCF, and BCF generations, respectively. A total of 27 plants with in homozygous state were identified in BCF generation and selfed to produce 27 BCF NILs. All the NILs were tested for leaf and stripe rust resistance at the seedling stage using seven and one f.sp. rust pathotypes. All the 27 NILs were found to be resistant to both leaf and stripe rust pathotypes. So, these NILs are designated to carry leaf and stripe rust resistance genes .