AI Article Synopsis
- The study utilized liquid chromatography tandem mass spectrometry to analyze an extract and identified 38 chemical peaks, pinpointing several cytochalasins.
- A new compound was discovered at the peak 540.26 [M + H], which was further purified and characterized as 19,20-epoxycytochalasin N1, a novel optical isomer.
- The compound demonstrated significant cytotoxicity, with IC values ranging from 1.34 to 19.02 μM, showcasing an efficient method for identifying new cytochalasin metabolites in crude extracts.
Article Abstract
The - extract was subjected to detailed liquid chromatography tandem mass spectrometry studies. A total of 38 peaks were annotated to / 508.26, / 510.28, / 524.26, / 526.28, / 540.26, / 542.27, and / 584.28 [M + H]. The accurate mass, mutually supported UV/vis spectra, and database search identified these compounds as cytochalasins. Systematic dereplication helped identify a peak at / 540.26 [M + H] as the new compound. Further, the identified compound was purified by high-performance liquid chromatography and characterized by 2D NMR to be 19,20-epoxycytochalasin N1, a new optical isomer of 19,20-epoxycytochalasin-N. It exhibited substantial cytotoxicity with IC values ranging from 1.34 to 19.02 μM. This study shows a fast approach for dereplicating and identifying novel cytochalasin metabolites in crude extracts.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404496 | PMC |
| http://dx.doi.org/10.1021/acsomega.2c03037 | DOI Listing |
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