Molecular Detection of in Isolated Fleas of Dogs in Meshkinshahr County, Northwest of Iran.

Iran J Parasitol

Department of Medical Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Published: January 2022

Background: Fleas (Insecta: Siphonaptera) are considered as highly specialized bloodsucking on mammals such as humans, livestock, dog, cat, rabbit, squirrels, rats, and mice. The desire for blood feeding from warm-blooded animals has led to becoming an intermediate host for some tapeworms like . The aim of this study was to detect larval infection in fleas of dogs living in Mesh-kinshahr County, northwest of Iran.

Methods: Fleas were collected from 42 dogs using brushing the hair in Meshkinshahr for one year (2014-2015). After the morphological study, fleas were preserved in 90% ethanol for molecular identification. After DNA extraction, the 28S ribosomal RNA gene (∼670 bp) of was amplified using specific primers. Finally, the PCR products were sequenced.

Results: Overs, 974 fleas were collected from the dogs. In the morphological study, three species , and were identified. PCR and sequence analysis results showed that 4 isolates were infected with . Also, no positive specimens were isolated from the other two species.

Conclusion: is reported as the most important species of fleas in transferring in that region.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9363253PMC
http://dx.doi.org/10.18502/ijpa.v17i2.9531DOI Listing

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