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Molecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative Colitis. | LitMetric
Background/aim : Ulcerative Colitis (UC) is an inflammatory bowel disease which is common in many areas of the world including Egypt. A lot of controversy regarding the pathogenesis of UC exist. The current study is an attempt to detect some pathogenic bacteria in UC patients.Materials And Methods : Endoscopic colonic biopsies obtained from 40 patients with ulcerative colitis and 20 controls were analyzed by means of real-time PCR technique for the presence of () and pathogenic ( which are positive for KPC and/or OXA-48.Results : All patients and control samples were negative for . Three of the 40 patient samples (7.5%) and none of the 20 controls were positive for with no significant difference between the two groups. KPC-positive were detected in 11 of the 40 patients (27.5%) and in none of the controls with a significant difference between the two groups (P=0.01). All patients and control samples were negative for OXA-48 positive E. coli.Conclusion : Although this study does not support the claim that and/or have a role in UC, it greatly suggests that pathogenic may be involved in one way or another in the course of UC.
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