Confined catalysis of MOF-818 nanozyme and colorimetric aptasensing for cardiac troponin I.

Talanta

Jiangsu Key Laboratory of Green Synthetic Chemistry for Functional Materials, School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou 221116, China. Electronic address:

Published: January 2023

Understanding the catalytic performance of nanozymes assembled in confined environment is an interesting topic. Herein, a three-dimensional nanozyme-catalytic nanoreactor was constructed by confining MOF-818 nanozyme in the pore of macroporous tungsten trioxide (p-WO). The catalytic activity of MOF-818 assembled in-situ for the oxidation of 3,5-Di-tert-butylcatechol (3,5-DTBC) could be regulated by changing the pore size of p-WO. Only when being confined in the pores of p-WO with an appropriate pore size, MOF-818 could exhibit high affinity towards 3,5-DTBC, and excellent catalytic activity for 3,5-DTBC oxidation, the catalytic rate constant k and Michaelis constant K were determined to be 31.47 s and 1.42 mM, respectively, and the maximum yield of 3,5-DTBC oxidation reached 95.2%. Furthermore, the as-constructed nanozyme-catalytic nanoreactor could be designed to construct a colorimetric aptasensor for selective determining cardiac troponin I based on the enzymatic inhibition effect and the exonuclease I-assisted target recycling signal amplification, which exhibited a good linear range of 50 fg mL - 100 ng mL, low detection limit of 18 fg mL, and was applied for human serum analysis with RSD less than 5.2% and the recoveries ranged from 95% to 107%.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2022.123830DOI Listing

Publication Analysis

Top Keywords

mof-818 nanozyme
8
cardiac troponin
8
nanozyme-catalytic nanoreactor
8
catalytic activity
8
pore size
8
35-dtbc oxidation
8
confined catalysis
4
mof-818
4
catalysis mof-818
4
nanozyme colorimetric
4

Similar Publications

MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.

Adv Healthc Mater

August 2024

Guangdong Provincial Key Laboratory of Urological Diseases, Guangdong Engineering Research Center of Urinary Minimally invasive surgery Robot and Intelligent Equipment, Guangzhou Institute of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, 510120, China.

There remains a lack of effective drugs to alleviate the kidney stones caused by oxidative stress and inflammatory damage. The MOF-818 nanozyme is utilized to lessen the generation of reactive oxygen species (ROS) effectively, restore the membrane potential of mitochondria, regulate the cell cycle, decrease cell death, hinder the recruitment of macrophages, and mitigate the release of inflammatory factors in macrophages. These effects are attributed to the nanozyme's ability to mimic the enzyme properties of catalase (CAT) and superoxide dismutase (SOD).

View Article and Find Full Text PDF

Background: Accurate and quick judgement of the food quality can protect the legitimate rights of consumers. Currently, nanozymes are widely employed in the rapid detection of food due to their stability and economy. The contents of bisphenol A and antioxidant can be used to measure the quality of beverages.

View Article and Find Full Text PDF

Self-Cascade Ce-MOF-818 Nanozyme for Sequential Hydrolysis and Oxidation.

Small

March 2024

The Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.

Article Synopsis
  • - The study focuses on developing a self-cascade nanozyme called Ce-MOF-818 that can efficiently perform sequential hydrolysis and oxidation reactions, addressing a significant gap in catalytic chemistry.
  • - Ce-MOF-818 is the first Ce(IV)-based heterometallic metal-organic framework created by coordinating cerium (Ce) and copper (Cu), synthesized through an innovative solvothermal method.
  • - The nanozyme shows impressive catalytic activities, outperforming traditional mixed nanozymes by about fourfold due to the close integration of its active sites, offering excellent potential for applications in catalytic processes.
View Article and Find Full Text PDF

MOF-On-MOF Dual Enzyme-Mimic Nanozyme with Enhanced Cascade Catalysis for Colorimetric/Chemiluminescent Dual-Mode Aptasensing.

Anal Chem

July 2023

Research Center for Intelligent and Wearable Technology, College of Textiles and Clothing, State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao 266071, China.

A novel metal-organic framework (MOF)-on-MOF dual enzyme-mimic nanozyme was designed as enhanced cascade signal amplification for the colorimetric and chemiluminescent (CL) dual-mode aptasensing. The MOF-on-MOF hybrid is composed of MOF-818 with catechol oxidase-like activity and iron porphyrin MOF [PMOF(Fe)] with peroxidase-like activity, called MOF-818@PMOF(Fe). MOF-818 can catalyze the 3,5-di--butylcatechol substrate and produce HO in situ.

View Article and Find Full Text PDF

Chronic wound is a common complication for diabetic patients, which entails substantial inconvenience, persistent pain, and significant economic burden to patients. However, current clinical treatments for diabetic chronic wounds remain unsatisfactory. A prolonged but ineffective inflammation phase in chronic wounds is the primary difference between diabetic chronic wounds and normal wounds.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!