In this study, the performance of a paper-based, screen-printed biofuel cell with mesoporous MgO-templated carbon (MgOC) electrodes was improved in two steps. First, a small amount of carboxymethyl cellulose (CMC) was added to the MgOC ink. Next, the cathode was modified with bilirubin prior to immobilizing the bilirubin oxidase (BOD). The CMC increased the accessibility of the mesopores of the MgOC, and subsequently, the performance of both the bioanode and biocathode. CMC also likely increased the stability of the electrodes. The pre-modification with bilirubin improved the orientation of the BOD, which facilitated direct electron transfer. With these two steps, an open circuit potential of 0.65 V, a maximal current density of 1.94 mA cm, and a maximal power density of 465 μW cm was achieved with lactate oxidase as bioanode enzyme and lactate as fuel. This is one of the highest reported performances for a biofuel cell.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420125 | PMC |
| http://dx.doi.org/10.1038/s41598-022-19052-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Engineering a Binding Peptide for Oriented Immobilization and Efficient Bioelectrocatalytic Oxygen Reduction of Multicopper Oxidases.
ACS Appl Mater Interfaces
December 2024
Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China.
Enzymatic fuel cells (EFCs) are emerging as promising technologies in renewable energy and biomedical applications, utilizing enzyme catalysts to convert the chemical energy of renewable biomass into electrical energy, known for their high energy conversion efficiency and excellent biocompatibility. Currently, EFCs face challenges of poor stability and catalytic efficiency at the cathodes, necessitating solutions to enhance the oriented immobilization of multicopper oxidases for improved heterogeneous electron transfer efficiency. This study successfully identified a surface-binding peptide (SBP, 13 amino acids) derived from a methionine-rich fragment (MetRich, 53 amino acids) in CueO through semirational design.
View Article and Find Full Text PDFAn oxygen-scavenging system without impact on DNA mechanical properties in single-molecule fluorescence experiments.
Nanoscale
December 2024
School of Physics, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
Oxygen scavenging systems (OSSs) are critical for dye stability in single-molecule fluorescence (SMF) experiments. However, the commonly used protocatechuic acid (PCA)/protocatechuate-3,4-dioxygenase (PCD) OSS alters DNA mechanical properties, limiting its applicability. To address this limitation, we examine the bilirubin oxidase (BOD) OSS, which had not been previously used in single-molecule experiments, alongside the pyranose oxidase and catalase (POC) OSS.
View Article and Find Full Text PDFMagnetohydrodynamic Enhancement of Biofuel Cell Performance.
Chemistry
November 2024
Univ. Bordeaux, CNRS, Bordeaux INP, ISM UMR 5255, 33607, Pessac, France.
Biofuel cells have become an interesting alternative for the design of sustainable energy conversion systems with multiple applications ranging from biosensing and bioelectronics to autonomously moving devices. However, as an electrochemical system, their performance is intimately related to mass transport conditions. In this work, the magnetohydrodynamic (MHD) effect is studied as an easy and straightforward alternative to enhance the performance of a biofuel cell based on the enzymes glucose oxidase (GOx) and bilirubin oxidase (BOD).
View Article and Find Full Text PDFConductance Channels in a Single-Entity Enzyme.
J Phys Chem Lett
October 2024
1 São Carlos Institute of Chemistry, University of São Paulo (USP), São Carlos, SP 13566-590, Brazil.
For a long time, the prevailing view in the scientific community was that proteins, being complex macromolecules composed of amino acid chains linked by peptide bonds, adopt folded structure with insulating or semiconducting properties, with high bandgaps. However, recent discoveries of unexpectedly high conductance levels, reaching values in the range of dozens of nanosiemens (nS) in proteins, have challenged this conventional understanding. In this study, we used scanning tunneling microscopy (STM) to explore the single-entity conductance properties of enzymatic channels, focusing on bilirubin oxidase (BOD) as a model metalloprotein.
View Article and Find Full Text PDFBiofuel cell-based self-powered immunosensor for detection of 17β-estradiol by integrating the target-induced biofuel release and biogate immunoassay.
Mikrochim Acta
July 2024
Functional Nanomaterial-Based Chemical and Biological Sensing Technology Innovation Team of Department of Education of Yunnan Province, Yunnan Minzu University, Kunming, 650504, People's Republic of China.
A novel biofuel cell (BFC)-based self-powered electrochemical immunosensing platform was developed by integrating the target-induced biofuel release and biogate immunoassay for ultrasensitive 17β-estradiol (E2) detection. The carbon nanocages/gold nanoparticle composite was employed in the BFCs device as the electrode material, through which bilirubin oxidase and glucose oxidase were wired to form the biocathode and bioanode, respectively. Positively charged mesoporous silica nanoparticles (PMSN) were encapsulated with glucose molecules as biofuel and subsequently coated by the negatively charged AuNPs-labelled anti-E2 antibody (AuNPs-Ab) serving as a biogate.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!