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Evaluation of the Immunomodulatory Effect of the Recombinant 14-3-3 and Major Antigen Proteins of against an Infection by . | LitMetric

AI Article Synopsis

  • - Strongyloidiasis is a neglected parasitic disease that poses significant health risks, especially in individuals with weakened immune systems, potentially leading to severe complications and high mortality rates.
  • - Researchers identified key proteins (14-3-3 and major antigen proteins) in the larvae of the parasite and produced them as recombinant proteins to study their role in immune response and potential for vaccine development.
  • - Despite vaccination efforts with these recombinant proteins in mice, results showed no protection against infection, but the rSs14-3-3 protein did alter the immune response, indicating it may act as an immunomodulator by increasing certain cytokine levels.

Article Abstract

Strongyloidiasis, caused by is a neglected parasitic disease that represents a serious public health problem. In immunocompromised patients, this parasitosis can result in hyperinfection or disseminated disease with high levels of mortality. In previous studies, the mRNAs encoding for the 14-3-3 and major antigen proteins were found to be expressed at high levels in L3 larvae, suggesting potential key roles in parasite-host interactions. We have produced them as recombinant proteins (rSs14-3-3 and rSsMA) in a bacterial protein expression system. The serum levels of anti-rSs14-3-3 and anti-rSsMA IgGs are increased upon infection with validating the use of the mouse model since the native 14-3-3 and MA proteins induce an immune response. Each recombinant protein was formulated in the adjuvant adaptation (ADAD) vaccination system and injected twice, subcutaneously, in CD1 mice that were experimentally infected with 3000 L3 to evaluate their protective and immunomodulatory activity. Our results, including the number of parthenogenetic females, number of eggs in stool samples and the analysis of the splenic and intestinal indexes, show that the vaccines did not protect against infection. The immunization with rSs14-3-3 induced changes in the cytokine profile in mice, producing higher expression of IL-10, TGF-β, IL-13 and TNF-α in the spleen, suggesting a Th2/Treg-type response with an increase in TNF-α levels, confirming its role as an immunomodulator.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9415175PMC
http://dx.doi.org/10.3390/vaccines10081292DOI Listing

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