A Comprehensive Analysis of the Myocardial Transcriptome in -Knockout Bama Xiang Pigs.

The gene is a transcription factor that regulates the expression of and affects muscle growth and development. However, its effect on the growth and development of the heart is still unknown. Emerging evidence suggests that long noncoding RNAs (lncRNAs) can regulate genes at the epigenetic, transcriptional, and posttranscriptional levels and play an important role in the development of eukaryotes. To investigate the function of in the cardiac development of pigs, we constructed the expression profiles of mRNAs and lncRNAs in myocardial tissue obtained from Bama Xiang pigs in the knockout group (-KO) and the wild-type group (-WT). A total of 248 differentially expressed genes (DEGs) and 209 differentially expressed lncRNAs (DELs) were detected, and 105 potential cis target genes of DELs were identified. The functional annotation analysis based on the Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) databases revealed two GO items related to muscle development by the cis target genes of DELs. Moreover, was the direct target gene of by ChIP-PCR experiment. Our results explored the mechanism and expression profile of mRNAs and lncRNAs of gene knockout on myocardium tissue development, mining the key candidate genes in that process like .