The aim of this study was to investigate the effect of triiodothyronine (T3) on tendon specific markers and cytokines expression of stem cells extracted from human tendons. Indeed, thyroid hormones have been reported to be protective factors, maintaining tendons' homeostasis, whereas tendinopathy is believed to be related to a failed healing response. Healthy and tendinopathic human tendons were harvested to isolate tendon stem/progenitor cells (TSPCs). TSPCs obtained from pathological samples showed gene expression and morphological modifications at baseline in comparison with cells harvested from healthy tissues. When cells were maintained in a medium supplemented with T3 (10 M), only pathological populations showed a significant upregulation of tenogenic markers (, , , ). Immunostaining revealed that healthy cells constantly released type I collagen, typical of tendon matrix, whereas pathological ones overexpressed and secreted type III collagen, typical of scarred and impaired tissue. Pathological cells also overexpressed pro- and anti-inflammatory cytokines, suggesting an impaired balance in the presence of T3, without STAT3 activation. Moreover, DKK-1 was significantly high in the culture medium of pathological cell cultures and was reversed by T3. This study opens perspectives on the complex biochemical alteration of cells from pathological tendons, which may lead to the chronic disease context with an impaired extracellular matrix.
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http://dx.doi.org/10.3390/cells11162545 | DOI Listing |
Jpn J Radiol
January 2025
Artificial Intelligence and Translational Imaging (ATI) Lab, Department of Radiology, School of Medicine, University of Crete, Voutes Campus, Heraklion, Greece.
Objective: Calcific tendinopathy, predominantly affecting rotator cuff tendons, leads to significant pain and tendon degeneration. Although US-guided percutaneous irrigation (US-PICT) is an effective treatment for this condition, prediction of patient' s response and long-term outcomes remains a challenge. This study introduces a novel radiomics-based model to forecast patient outcomes, addressing a gap in the current predictive methodologies.
View Article and Find Full Text PDFArch Orthop Trauma Surg
January 2025
Abteilung für Plastische und Handchirurgie UniversitätsCentrum für Orthopädie, Unfall- & Plastische Chirurgie, Universitätsklinikum Carl Gustav Carus an der Technischen Universität Dresden, Dresden, Germany.
Background: Kirner deformity is a rare anomaly of the little finger in adolescents, characterized by a deformity of the distal phalanx and a radiologically L-shaped epiphysis, along with palmar and radial angulation of the distal phalanx. Due to the rarity of these pathological findings, there are no systematic literature reviews available. This work serves as an overview of the clinical presentation, frequency and age distributions, as well as possible conservative and surgical treatment options.
View Article and Find Full Text PDFAlzheimers Dement
December 2024
Koc University, Department Biology and Genetics, Istanbul, Turkey.
Background: Valosin Containing Protein (VCP) mutations are responsible some genetic etiologies of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD).
Method: A 67-year-old, male patient, applied to the clinic due to behavioral changes and difficulty swallowing. According the patient history it was reported that his first complaint started 6 years ago (at the age of 61).
Cureus
December 2024
Otorhinolaryngology Department, Unidade Local de Saúde de São João, Porto, PRT.
Objectives The aim of this anatomical study was to analyze distances and anatomical relations between the lower cranial nerves and important neck landmarks. Methods Anatomical study based on neck dissection in Thiel-embalmed cadavers. Anatomical relations and distances between the vagus (X), accessory (XI), and hypoglossal (XII) nerves and important neck landmarks were registered and compared.
View Article and Find Full Text PDFCureus
December 2024
Department of Science of Physical Functions, Division of Oral Medicine, Kyushu Dental University, Kitakyushu, JPN.
Objective Masticatory muscle tendon-aponeurosis hyperplasia (MMTAH) is a recently identified condition characterized by restricted mouth opening due to hyperplasia of the temporalis muscle tendon and masseter muscle aponeurosis. This study examines the treatment and clinical course of patients with MMTAH who underwent surgery at our hospital. Subjects and methods The study included 14 patients (four males and 10 females; mean age: 33.
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