AI Article Synopsis
- - This study introduced a new method for assessing blood coagulation using blood-impedance-magnitude measurement, identifying two important biomarkers linked to fibrin formation and clot retraction during the process.
- - A combination of confocal microscopy and impedance analysis provided a detailed visual and quantitative understanding of coagulation events, confirming the relevance of the identified biomarkers.
- - The technique holds promising applications for monitoring coagulation on various materials, particularly in relation to artificial implants and cardiovascular prostheses, potentially enabling real-time material characterization.
Article Abstract
This study focused on a coagulation assessment based on the novel technique of blood-impedance-magnitude measurement. With the impedance characterization of recalcified human blood, it was possible to identify two significative biomarkers (i.e., measurable indicators) related to fibrin formation (1st marker) and clot retraction (2nd marker). The confocal microscopy of clotting blood provided a complete visual analysis of all the events occurring during coagulation, validating the significance of the impedance biomarkers. By analyzing the impedance phase angle () of blood during coagulation, as well as those of the clot and serum expelled after retraction, it was possible to further clarify the origin of the 2nd marker. Finally, an impedance-magnitude analysis and a rotational thromboelastometry test (ROTEM) were simultaneously performed on blood sampled from the same donor; the results pointed out that the 1st marker was related to clotting time. The developed technique gives rise to a comprehensive and evolutive insight into coagulation, making it possible to progressively follow the whole process in real time. Moreover, this approach allows coagulation to be tested on any materials' surface, laying the ground for new studies related to contact coagulation, meaning, thrombosis occurring on artificial implants. In a near future, impedance spectroscopy could be employed in the material characterization of cardiovascular prostheses whose properties could be monitored in situ and/or online using effective biomarkers.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9404805 | PMC |
| http://dx.doi.org/10.3390/biomedicines10081833 | DOI Listing |
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