AI Article Synopsis
- Richard Carter's 2005 method, "linkage group selection", revolutionized malaria genetics by using bulk progeny pools to quickly map traits like drug resistance.
- This method also introduced "bulk segregant" strategies, which are now popular in various microbes, including yeast and several pathogens.
- Recent advances allow genetic crosses of human malaria parasites in humanized mice, opening up further research opportunities for mapping diverse traits in malaria.
Article Abstract
In 2005 Richard Carter's group surprised the malaria genetics community with an elegant approach to rapidly mapping the genetic basis of phenotypic traits in rodent malaria parasites. This approach, which he termed "linkage group selection", utilized bulk pools of progeny, rather than individual clones, and exploited simple selection schemes to identify genome regions underlying resistance to drug treatment (or other phenotypes). This work was the first application of "bulk segregant" methodologies for genetic mapping in microbes: this approach is now widely used in yeast, and across multiple recombining pathogens ranging from Aspergillus fungi to Schistosome parasites. Genetic crosses of human malaria parasites (for which Richard Carter was also a pioneer) can now be conducted in humanized mice, providing new opportunities for exploiting bulk segregant approaches for a wide variety of malaria parasite traits. We review the application of bulk segregant approaches to mapping malaria parasite traits and suggest additional developments that may further expand the utility of this powerful approach.
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| http://dx.doi.org/10.1016/j.parint.2022.102653 | DOI Listing |
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