Stable sulphur isotope (S) ratios in bird feathers from India indicate strong segregation between the Himalaya and Gangetic plain, and the rest of India.

Although stable hydrogen (H) and carbon (C) isotopic compositions of bird feathers have been increasingly used to understand the migration of birds through assignment of individuals to established isoscapes, far less is known about the structure of feather isoscapes based on stable sulphur isotope (S) assays. While H isoscapes have been used to investigate the migratory origins of the Globe Skimmer () dragonfly in India, no isotope studies have been applied to avian migration patterns in that region. Here, based on museum collections, we report the feather S values of 13 avian species that included marine birds with expected S enriched feathers, resident birds throughout India and a single migratory species, Bar-headed Goose (). Feathers from the marine birds were the most enriched (20.6 ± 1.2 ‰,  = 10; range = 4.4 ‰) in S but terrestrial resident species also showed unexpectedly high S values (11.7 ± 4.8 ‰,  = 74; range = 19.9 ‰) throughout most of India but the Himalayan region and Gangetic plain typically showed S values less than 6.4 ‰. Our results show the utility of feather S studies to decipher avian and other animal migration between these two regions.