Genetic diversity of rhizobia isolated from nodules of Trigonella foenum-graecum L. (fenugreek) cultivated in Northwestern Morocco.

Arch Microbiol

Laboratory of Biotechnological Valorization of Microorganisms, Faculty of Sciences and Techniques of Tangier, Abdelmalek Essaadi University, Tétouan, Morocco.

Published: August 2022

The present work aimed to characterize rhizobia nodulating Trigonella foenum-graecum L. (fenugreek) cultivated in six different geographical locations in Northwestern Morocco. Forty-seven rhizobial isolates from nodules of Trigonella foenum-graecum were grouped into thirteen clusters using the Rep-PCR technique. The phylogenetic analysis based on 16S rRNA gene sequences of 13 groups showed that all representative strains were closely related to members of the genus Ensifer (syn. Sinorhizobium) of the Alphaproteobacteria. All the representative strains shared 100% similarity with Ensifer medicae WSM419. NodC and nifH gene analysis revealed a close phylogenetic relationship of the representative strains with those of the strains belonging to the symbiovar meliloti. Furthermore, nodulation ability of our rhizobial strains was efficient in their host plant (Trigonella foenum-graecum L.).

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http://dx.doi.org/10.1007/s00203-022-03189-xDOI Listing

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