AI Article Synopsis
- The study introduces a novel assay that uses fluorogenic RNA aptamers to detect uracil-DNA glycosylase (UDG) without needing labels.
- By modifying the transcription process with dU substitution, this method only needs one probe to both sense and amplify UDG signals, reaching a detection limit of 6.3 × 10 U mL.
- This approach can also be utilized for screening potential UDG inhibitors and assessing UDG activity in various cell types.
Article Abstract
We demonstrate for the first time the utilization of fluorogenic RNA aptamers for label-free uracil-DNA glycosylase (UDG) assay. Through rationally engineering the transcription machine with dU substitution, this assay requires only a single probe to simultaneously sense and amplify the UDG signal, achieving a low detection limit of 6.3 × 10 U mL. Moreover, it can be applied for screening UDG inhibitors and measuring endogenous UDG activity in different cells.
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