The combination of RNA-seq transcriptomics and data-independent acquisition proteomics reveals the mechanisms underlying enhanced salt tolerance by the gene in [L.] Merr.

[L.] Merr. is one of the three most economically important species due to its strong salt tolerance and wide application. However, the molecular mechanisms regulating salt tolerance in remain unknown. The protein disulfide isomerase of was obtained by salt stress screening with yeast cells, and its expression was significantly upregulated after salt stress. Based on the obtained overexpression transgenic plants, we carried out salt tolerance identification and found that can significantly enhance the salt tolerance of . Root samples of transgenic and wild-type plants were collected at 0 and 24 h after salt treatments for RNA-seq and data-independent acquisition (DIA) proteome sequencing. Combined analysis of the transcriptome and proteome revealed that may enhance the salt tolerance of by regulating , , α, , and . This research presents the molecular regulatory mechanism of the gene in for resistance to salt stress and facilitates the use of molecular breeding to improve the salt tolerance of grasses.