Targeted Isolation of Anti-inflammatory Lignans from by Molecular Networking Approach.

Herein, the isolation of secondary metabolites from the aerial parts of guided by HPLC-MS and molecular networking analyses is reported. Twenty-two known compounds were dereplicated. Three new lignans (aequilabrines A-C (-)) and three known compounds (lariciresinol-4'-β-glucose (), roseoside (), and allantoin ()) were obtained. The anti-inflammatory activity of compounds - was evaluated by inhibiting the nitric oxide production (NO) and pro-inflammatory activity on the cytokine IL-1β. Compounds and showed significant inhibitory activity against NO production, with IC values of 9.1 and 7.3 μM, respectively. The maximum inhibition of IL-1β production was 23.5% (), 27.3% (), and 32.5% ().