AI Article Synopsis
- In the social amoeba Dictyostelium discoideum, the polyketide MPBD plays a vital role in regulating cAMP signaling for cell aggregation and spore maturation.
- The polyketide synthase StlA is responsible for the biosynthesis of MPBD and is conserved across major groups of social amoebas.
- Research in Polysphondylium violaceum showed that StlA is essential for cell aggregation but not for spore maturation, and MPBD can reverse aggregation defects in StlA knockout mutants while chemotaxis remains unaffected.
Article Abstract
In the social amoeba Dictyostelium discoideum, the polyketide MPBD (4-methyl-5-pentylbenzene-1,3-diol) regulates the gene expressions of cAMP signaling to make cells aggregation-competent and also induces spore maturation. The polyketide synthase StlA is responsible for MPBD biosynthesis in D. discoideum and appears to be conserved throughout the major groups of the social amoeba (Dictyostelia). In this study, we analyzed the function of StlA in Polysphondylium violaceum by identifying the gene sequence and creating the knockout mutants. We found that Pv-stlA- mutants had defects only in cell aggregation but not in spore maturation, indicating that the function of StlA in inducing spore maturation is species-specific. We also found that MPBD could rescue the aggregation defect in Pv-stlA- mutants whereas the mutants normally exhibited chemotaxis to their chemoattractant, glorin. Our data suggest that StlA is involved in inducing aggregation in P. violaceum by acting on signaling pathways other than chemotaxis in P. violaceum.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/bbb/zbac144 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Morphology and Phylogenetic Analysis of Henneguya sp. Infecting the Orange-Spotted Snakehead (Channa Aurantimaculata) from Tamil Nadu, India.
Acta Parasitol
January 2025
ICAR-Central Island Agricultural Research Institute, Andaman & Nicobar Islands, Port Blair, India.
Purpose: Henneguya sp. is a crucial myxosporean parasite known to cause milky flesh or tapioca disease in the freshwater fish population, leading to heavy mortality. Studies to investigate its host range and to monitor their prevalence in wild and aquacultured fish are necessary.
View Article and Find Full Text PDFFirst Report of Postharvest Fruit Rot on Passion Fruit () caused by in the U.S.A.
Plant Dis
January 2025
University of Florida Tropical Research and Education Center, Plant Pathology, 1615 SE 23rd Way, Homestead, Florida, United States, 33031-3314;
The commercial production of passion fruit is geographically limited (California, Florida, and Hawaii), but the development of cold-tolerant varieties could expand it beyond warm-climate states (Stafne et.al. 2023).
View Article and Find Full Text PDFSeparation of life stages within anaerobic fungi (Neocallimastigomycota) highlights differences in global transcription and metabolism.
Fungal Genet Biol
December 2024
University of California, Santa Barbara, Department of Chemical Engineering, Santa Barbara, CA 93106, USA; Joint BioEnergy Institute (JBEI), Emeryville, CA, 94608, United States. Electronic address:
Anaerobic gut fungi of the phylum Neocallimastigomycota are microbes proficient in valorizing low-cost but difficult-to-breakdown lignocellulosic plant biomass. Characterization of different fungal life stages and how they contribute to biomass breakdown are critical for biotechnological applications, yet we lack foundational knowledge about the transcriptional, metabolic, and enzyme secretion behavior of different life stages of anaerobic gut fungi: zoospores, germlings, immature thalli, and mature zoosporangia. A Miracloth-based technique was developed to enrich cell pellets with zoospores - the free-swimming, flagellated, young life stage of anaerobic gut fungi.
View Article and Find Full Text PDFA glucomannan from defatted Ganoderma lucidum spores: structural characterization and immunomodulatory activity via activating TLR4/MyD88/NF-κB signaling pathway.
Int J Biol Macromol
December 2024
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China. Electronic address:
Ganoderma lucidum spores are tiny mature germ cells ejected from the abaxial side of the pileus and were responsible for multiple pharmacological properties. The defatted G. lucidum spores are the byproducts after the extraction of G.
View Article and Find Full Text PDFSaprolegniasis is one of the most dangerous fungal diseases of fish, causing significant mortality in fish hatcheries and young ones. The present study aimed to isolate and characterize the causative fungus from fingerlings of Pangasianodon hypophthalmus cultured intensively in freshwater cages in Indian reservoirs and to determine minimum inhibitory concentrations of different antifungal compounds against the fungal hyphae and zoospores. The fungal isolates grown on potato dextrose agar showed an abundance of gemmae, elongated mycelia, non-septate hyphae, primary zoospores, mature zoosporangia with numerous zoospores, cysts with bundles of long hairs and were further identified as Saprolegnia parasitica following PCR amplification and sequencing of internal transcribed spacer region.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!