The use of deep eutectic solvents (DESs) has great prospects because of the green and efficient characteristics, which can be used for developing analytical methods for foods. In this research, assisted by ultrasonic waves, a liquid-liquid microextraction detection method combined with gas chromatography was established for three anaesthetics (eugenol, isoeugenol, and methyl isoeugenol) in aquatic food. The processing conditions including the components, ratio of hydrogen bond acceptor and hydrogen bond donor, DES volume, ultrasonic time, and pH were evaluated and optimised to improve the extraction efficiency, which was based on the DES structures and properties. In-house method validation was carried out by applying to real samples. A Thymol: levulinic acid DES (with a molar ratio of 1:2) was used as the extractant and the recoveries were as high as 93-101% for eugenol, 90-100% for methyl isoeugenol, and 86-94% for isoeugenol with RSDs <5% under optimum conditions. The limit of detection and quantification of the eugenol compounds were 0.08-0.10 μg/mL and 0.26-0.33 μg/mL, respectively. The method has green credentials and comparable LOD to homologous apparatus, which can be used for the determination of eugenol components in aquatic food.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/19440049.2022.2112764 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sedative-Hypnotic Effect and Mechanism of Carbon Nanofiber Loaded with Essential Oils of ( Hort.) and Finger Citron ( L. var. ) on Mice Models of Insomnia.
Biomolecules
September 2024
State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
(1) Background: Insomnia is a neurological illness that poses a significant threat to both physical and mental health. It results in the activation of neuroglial cells, heightened neuroinflammation, oxidative stress, and disruptions in the Hypothalamic-Pituitary-Adrenal (HPA) axis. (CX) and Finger citron (FC) are frequently utilized botanicals for addressing sleeplessness.
View Article and Find Full Text PDFDiscovery of a new class of bacterial heme-containing CC cleaving oxygenases.
N Biotechnol
November 2024
Molecular Enzymology, University of Groningen, Nijenborgh 4, Groningen 9747AG, the Netherlands. Electronic address:
Previously, some bacteria were shown to harbour enzymes capable of catalysing the oxidative cleavage of the double bond of t-anethole and related compounds. The cofactor dependence of these enzymes remained enigmatic due to a lack of biochemical information. We report on catalytic and structural details of a representative of this group of oxidative enzymes: t-anethole oxygenase from Stenotrophomonas maltophilia (TAO).
View Article and Find Full Text PDFGold nanocluster-based ratiometric fluorescence immunoassay for broad-spectrum screening of five eugenols.
Anal Chim Acta
June 2024
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China. Electronic address:
Background: Eugenol compounds (EUGs), which share chemical similarities with eugenol, belong to a group of phenolic compounds primarily found in clove oil. They are highly valued by fish dealers due to their exceptional anesthetic properties, playing a crucial role in reducing disease incidence and mortality during the transportation of live fish. Despite their widespread use, the safety of EUGs remains a contentious topic, raising concerns about the safety of aquatic products.
View Article and Find Full Text PDFDevelopment of broad-spectrum immunoassay with monoclonal antibody to detect five eugenols and study of their molecular recognition mechanism.
Food Chem X
June 2024
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
In this study, three eugenol fragment-containing haptens were synthesized, and a monoclonal antibody (mAb) selective for five commonly-found eugenol compounds (EUGs, i.e., eugenol, isoeugenol, methyl eugenol, methyl isoeugenol, and acetyl isoeugenol) was obtained.
View Article and Find Full Text PDFChemical Variation and Environmental Influence on Essential Oil of .
Molecules
January 2023
College of Forestry, Jiangxi Agricultural University, East China Woody Fragrance and Flavor Engineering Research Center of National Forestry and Grassland Administration, Nanchang 330045, China.
is a traditional aromatic plant used to produce linalool and borneol flavors in southern China; however, its leaves also contain many other unutilized essential oils. Herein, we report geographic relationships for the yield and compositional diversity of essential oils. The essential oils of 974 individual trees from 35 populations in 13 provinces were extracted by hydrodistillation and analyzed qualitatively and quantitatively by gas chromatography-mass spectrometry and gas chromatography-flame ionization detection, respectively.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!