Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting results showed that the minimum detection limit of the 4 filter papers was 1 × 10 parasites/L, and the positive rates of 20 PCR kits ranged from 0% to 100%. PCR results were satisfactory for detecting () and () in archived DBS samples and () in fresh pet DBS samples. Our results provided a useful reference for the detection of blood pathogens with DBS samples and direct PCR, especially for screening the cost-efficacy combination of filter paper and PCR kit in resource-limited areas.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392634 | PMC |
| http://dx.doi.org/10.1155/2022/5715436 | DOI Listing |
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