AI Article Synopsis
- The study investigated how epigallocatechin gallate (EGCG) affects the growth of Shewanella putrefaciens and its ability to form biofilms.
- EGCG was found to have a minimum inhibitory concentration (MIC) of 160 μg/mL, showing effective antimicrobial activity and damaging the bacterial cell wall and membrane, as confirmed by microscopy.
- Additionally, lower concentrations of EGCG led to decreased bacterial movement and increased oxidative stress, which unexpectedly resulted in higher biofilm formation, suggesting its potential use as a natural preservative for fish storage.
Article Abstract
The study was to evaluate the antimicrobial impacts and biofilm influences on epigallocatechin gallate (EGCG) against Shewanella putrefaciens ATCC 8071. The minimum inhibitory concentration (MIC) of EGCG on S. putrefaciens was 160 μg mL. The growth curve exhibited that EGCG had a good antimicrobial activity. EGCG caused damages to the bacterial cell wall and membrane based the intracellular component leakage and cell viability analysis. The damage to the membrane integrity by EGCG has been confirmed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM shows deformation of shape, TEM shows cell membrane and wall damage, and the leakage of cytoplasmic material. The treatment with EGCG at 0.25× and 0.5× MIC resulted in decreased motility and elevated levels of oxidative stress, leading to an increase in biofilm formation. These results demonstrated that EGCG may be used as a natural preservative to reduce S. putrefaciens in fish during cold storage.
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| http://dx.doi.org/10.1007/s00284-022-02978-3 | DOI Listing |
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