Department of Biological Sciences and Bioengineering, Indian Institute of Technology-Kanpur; The Mehta Family Centre for Engineering in Medicine, Indian Institute of Technology-Kanpur;
Published: August 2022
AI Article Synopsis
Article Abstract
The IFP, present in the knee joint, serves as a promising source of MSCs. The IFP is an easily accessible tissue as it is routinely resected and discarded during arthroscopic procedures and knee replacement surgeries. Additionally, its removal is associated with minimal donor site morbidity. Recent studies have demonstrated that IFP-MSCs do not lose their proliferation capacity during in vitro expansion and have age-independent osteogenic differentiation potential. IFP-MSCs possess superior chondrogenic differentiation potential compared to bone marrow-derived MSCs (BMSCs) and adipose-derived stem cells (ADSCs). Although these cells are easily obtainable from aged and diseased patients, their effectiveness is limited. Hence, using IFP-MSCs from healthy donors is important to determine their efficacy in biomedical applications. As access to a healthy human donor is challenging, animal models could be a better alternative to enable fundamental understanding. Large animals such as dogs, horses, sheep, and goats play a crucial role in translational research. Amongst these, the goat could be a preferred model since the stifle joint of the goat has the closest anatomy to the human knee joint. Moreover, goat-IFP can fulfill the higher MSC numbers needed for tissue regeneration applications. Furthermore, low cost, availability, and compliance with the 3R principles for animal research make them an attractive model. This study demonstrates a simple protocol for isolating IFP-MSCs from the stifle joint of goats and in vitro culture conditions for their expansion and differentiation. The aseptically isolated IFP from the goat was washed, minced, and digested enzymatically. After filtration and centrifugation, the collected cells were cultured. These cells were adherent, had MSCs-like morphology, and demonstrated remarkable clonogenic ability. Further, they differentiated into adipogenic, chondrogenic, and osteogenic lineages, demonstrating their multipotency. In conclusion, the study demonstrates the isolation and expansion of MSCs, which show potential in tissue engineering and regenerative medicine applications.
The purpose of this study was to identify the effects of four heights of cavaletti exercise on canine kinematics and kinetics. Eight dogs had kinematics and kinetics data collected whilst completing cavaletti pole exercises at four different heights (floor, mid-metatarsal, hock, and stifle). Dogs had anatomical markers placed on bony landmarks of the fore and hindlimb joints.
OrtopediaVet Veterinary Clinic, Dr. Eloy Curuci and team. Volta Redonda Street, 670 - Campo Belo, São Paulo, State of São Paulo, Brazil, Zip code 04608-011.
Objectives: There is a lack of literature regarding the surgical management of high-grade patellar luxation in cats. Among the available options, corrective osteotomies are suitable for correcting severe bone deformities. Therefore, this study aimed to report on the surgical management of grade IV medial patellar luxation (MPL) through tibial corrective osteotomies in two cats.
Objectives: The aim of this study was to assess the complication rates and long-term outcomes, as well as to refine case selection criteria for cats undergoing trochlear ridge augmentation (TRA) using an ultra-high molecular weight polyethylene implant (UHMWPEI) for the treatment of patellar luxation (PL).
Methods: The clinical records from two referral veterinary hospitals were searched for cases that fitted defined inclusion criteria. A short-term assessment was based on examination and radiography at 8 weeks postoperatively.
Amidst escalating environmental challenges, the imperative for sustainable development and environmental preservation has become central in contemporary discourse. Where the consumption of fossil fuels poses a significant threat to the global sustainability, the global shift towards renewable energy sources is recognized as a key strategy for nations to confront climate change, enhance economic prosperity, and contribute to global sustainability by promoting green economic growth. In this context, our study makes a momentous contribution to the existing disclosure by investigating the impact of renewable and non-renewable energy consumption on the green economic growth of, not only in the implementing country, but also on the surrounding economies across the South Asian Region.
Objective: The goal of this study was to investigate the effect of recording angle on the accuracy of 2-D Kinovea-based kinematic motion analysis (KMA) compared to 3-D KMA in dogs.
Methods: In this prospective study, 3-D marker-based KMA (VICON-Nexus, version 2.12.
