Unlabelled: In the gastrointestinal tract, some dietary carbohydrates, such as xylose, raffinose and arabinose, are able to stimulate the growth of and . In this study, the growth rate of Ren in raffinose was 0.91 ± 0.03 h, which was higher than that in glucose (0.83 ± 0.01 h). However, limited information is available on specific transporters and glycoside hydrolases responsible for raffinose uptake and catabolism in Transcriptomic analysis revealed the differential expression of 236 genes (∣logFoldChange∣ > 0.8) in response to raffinose, which were mainly associated with raffinose transport, raffinose hydrolysis, galactose metabolism and pyruvate metabolism. Notably, gene encoding lactose/raffinose permease was 101.86-fold up-regulated. Two α-galactosidase gene and were 117.82-fold and 2.66-fold up-regulated, respectively. To further investigate the role of these genes in raffinose utilization, insertional inactivation was performed using the pORI28-pTRK669 system. The growth assay of these mutants in modified MRS containing 2% (w/v) raffinose indicated that RafP played an important role in raffinose transport and GalA1 was the primary enzyme involved in raffinose hydrolysis. To our knowledge, this is the first report on the molecular mechanism of raffinose utilization in
Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03280-6.
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| http://dx.doi.org/10.1007/s13205-022-03280-6 | DOI Listing |
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