Green synthesis and characterization of silver nanoparticles using leaf extract and evaluation of its antioxidant and antimicrobial activity.

This study emphasizes the production of eco-friendly silver nanoparticles from a medicinal plant extract of and investigated its antioxidant and antimicrobial activity. Phytochemical screening of leave extract was carried out and observed to contain some fundamental phyto-reducing agents such as reducing sugar, proteins, and alkaloids. The green synthesized AgNPs (ML-AgNPs) were characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), transmission emission microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Energy dispersive X-ray analysis (EDX). Thermo gravimetric analysis (TGA) was performed on the synthesized ML-capped AgNPs to determine the thermal stability and the formation of the green synthesized AgNPs. The formation of AgNPs was confirmed by the UV-vis absorption spectra, which showed an absorption band at 420 nm. The morphology of ML extract-mediated AgNPs was mostly spherical and rough-edged crystallite nanostructures, with an average particle size of 11 nm. The FTIR analyses revealed distinctive functional groups which were directly involved in the synthesis and stability of AgNPs. The crystallite size was 8.79 nm, with four intense peaks at 2 angles of 38°, 44°, 64°, and 77°. At an energy level of 3.4 keV, a significant signal was observed indicating the production of thermally stable and pure crystallite AgNPs. The antioxidant property of green synthesized ML-AgNPs was determined to be 40% higher than that of crude leaf extract. The ability of green synthesized ML-AgNPs to scavenge free radicals also increased in the order of OH < NO < HO. The ML-AgNPs have strong activities with a maximum against and a minimum with