Prosthetic implants of expanded polytetrafluoroethylene (ePTFE) in the cardiovascular system have a high failure rate over the long term because of thrombosis and intimal hyperplasia. Although multiple surface modification methods have been applied to improve the anti-thrombotic and endothelialization abilities of ePTFE, none have delivered outstanding results . Our previous study combined heparin/collagen multilayers and REDV peptides to modify ePTFE, and the results showed that modification ePTFE with heparin/collagen-REDV can promote the cytocompatibility and antiplatelet property. This study illustrated the physical change, selective endothelial cells capture ability, and performance in further. The physical test demonstrated that this modification improved the hydrophilicity, flexibility and strength of ePTFE. A competition experiment of co-cultured endothelial cells and vascular smooth muscle cells verified that the heparin/collagen-REDV modification had high specificity for endothelial cell capture. A rabbit animal model was constructed to evaluate the performance of modified ePTFE implanted in the right ventricular outflow tract. The results showed that heparin/collagen-REDV modification was safe, promoted endothelialization, and successfully achieved regional anti-thrombosis without influencing body-wide coagulation function. The pathologic manifestations and mRNA expression pattern in tissues in contact with modified ePTFE indicated that this modification method may reduce M2-type macrophage infiltration and the expression of genes related to immune and inflammatory responses. The heparin/collagen-REDV modification may lower the incidence of complications related to ePTFE implantation and has good prospects for clinical use.
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http://dx.doi.org/10.3389/fbioe.2022.916931 | DOI Listing |
Front Bioeng Biotechnol
August 2022
Shanghai Key Laboratory of Cardiovascular Disease, Department of Cardiovascular Center, Children's Hospital of Fudan University, Shanghai, China.
Prosthetic implants of expanded polytetrafluoroethylene (ePTFE) in the cardiovascular system have a high failure rate over the long term because of thrombosis and intimal hyperplasia. Although multiple surface modification methods have been applied to improve the anti-thrombotic and endothelialization abilities of ePTFE, none have delivered outstanding results . Our previous study combined heparin/collagen multilayers and REDV peptides to modify ePTFE, and the results showed that modification ePTFE with heparin/collagen-REDV can promote the cytocompatibility and antiplatelet property.
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August 2018
Department of Cardiovascular Center, Children's Hospital of Fudan University, Shanghai, China.
Expanded polytetrafluoroethylene (ePTFE) prosthetic valves have been widely used in clinical applications in Asian countries. However, these valves still have limits with regard to thrombosis, neointimal hyperplasia, restenosis, and valvar vegetation. The achievement of in situ endothelialization on implant materials is a promising way to overcome those limits.
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