Cable bacteria (CB) are non-isolated filamentous bacteria in the family of , known for fostering centimeter-long electron transfer in sediments with pronounced redox zonation. This protocol details steps to extract CB filaments from cultured natural sediment, inoculate autoclaved sediment with extracted filaments, and subsequently evaluate the growth and enrichment of CB. We also describe the approaches for collecting suitable sediment, preparing autoclaved sediment, and manufacturing glass needles and hooks for the extraction of CB.
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http://dx.doi.org/10.1016/j.xpro.2022.101604 | DOI Listing |
bioRxiv
December 2024
Duke University School of Medicine, Department of Molecular Genetics and Microbiology, Duke Center for Virology, Durham, NC, USA.
Herpesviruses, including the oncogenic Epstein-Barr Virus (EBV), must bypass host DNA sensing mechanisms to establish infection. The first viral latency protein expressed, EBNA-LP, is essential for transformation of naïve B cells, yet its role in evading host defenses remains unclear. Using single-cell RNA sequencing of EBNA-LP-Knockout (LPKO)-infected B cells, we reveal an antiviral response landscape implicating the 'speckled proteins' as key restriction factors countered by EBNA-LP.
View Article and Find Full Text PDFAnal Chem
December 2024
State Key Laboratory of Optical Fiber and Cable Manufacture Technology, Guangdong Key Laboratory of Integrated Optoelectronics Intellisense, Department of EEE, Southern University of Science and Technology, Shenzhen 518055, China.
Raman spectroscopy enables label-free clinical diagnosis in a single step. However, identifying an individual carrying a specific disease from people with a multi-disease background is challenging. To address this, we developed a Raman spectral implicit feature augmentation with a Raman Intersection, Union, and Subtraction augmentation strategy (RIUS).
View Article and Find Full Text PDFmSystems
December 2024
Center for Electromicrobiology, Department of Biology, Aarhus University, Aarhus, Denmark.
Unlabelled: Cable bacteria, filamentous sulfide oxidizers that live in sulfidic sediments, are at times associated with large flocks of swimming bacteria. It has been proposed that these flocks of bacteria transport electrons extracellularly to cable bacteria via an electron shuttle intermediate, but the identity and activity of these bacteria in freshwater sediment remain mostly uninvestigated. Here, we elucidate the electron exchange capabilities of the bacterial community by coupling metagenomics and metatranscriptomics to 16S rRNA amplicon-based correlations with cable bacteria over 155 days.
View Article and Find Full Text PDFFEMS Microbiol Rev
December 2024
School of Chemistry, Monash University, Clayton, Australia.
Cable bacteria are a unique type of filamentous microorganism, which can grow up to centimetres long and are capable of long-distance electron transport over their entire lengths. Due to their unique metabolism and conductive capacities, the study of cable bacteria has required technical innovations, both in adapting existing techniques and developing entirely new ones. This review discusses the existing methods used to study eight distinct aspects of cable bacteria research, including the challenges of culturing them in laboratory conditions, performing physical and biochemical extractions, and analysing the conductive mechanism.
View Article and Find Full Text PDFJ Int Med Res
December 2024
Professor of Clinical Medicine, Division of Infectious Diseases, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
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