Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria.

STAR Protoc

College of Earth, Ocean, and Atmospheric Science, Oregon State University, Corvallis, OR 97333, USA.

Published: September 2022

Cable bacteria (CB) are non-isolated filamentous bacteria in the family of , known for fostering centimeter-long electron transfer in sediments with pronounced redox zonation. This protocol details steps to extract CB filaments from cultured natural sediment, inoculate autoclaved sediment with extracted filaments, and subsequently evaluate the growth and enrichment of CB. We also describe the approaches for collecting suitable sediment, preparing autoclaved sediment, and manufacturing glass needles and hooks for the extraction of CB.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389416PMC
http://dx.doi.org/10.1016/j.xpro.2022.101604DOI Listing

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