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Single-cell transcriptomics and cell-specific proteomics reveals molecular signatures of sleep. | LitMetric

Single-cell transcriptomics and cell-specific proteomics reveals molecular signatures of sleep.

Commun Biol

Department of Systems Pharmacology & Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Published: August 2022

AI Article Synopsis

Article Abstract

Every day, we sleep for a third of the day. Sleep is important for cognition, brain waste clearance, metabolism, and immune responses. The molecular mechanisms governing sleep are largely unknown. Here, we used a combination of single-cell RNA sequencing and cell-type-specific proteomics to interrogate the molecular underpinnings of sleep. Different cell types in three important brain regions for sleep (brainstem, cortex, and hypothalamus) exhibited diverse transcriptional responses to sleep need. Sleep restriction modulates astrocyte-neuron crosstalk and sleep need enhances expression of specific sets of transcription factors in different brain regions. In cortex, we also interrogated the proteome of two major cell types: astrocytes and neurons. Sleep deprivation differentially alters the expression of proteins in astrocytes and neurons. Similarly, phosphoproteomics revealed large shifts in cell-type-specific protein phosphorylation. Our results indicate that sleep need regulates transcriptional, translational, and post-translational responses in a cell-specific manner.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9391396PMC
http://dx.doi.org/10.1038/s42003-022-03800-3DOI Listing

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