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Advances in the exact nuclear Overhauser effect 2018-2022. | LitMetric

Advances in the exact nuclear Overhauser effect 2018-2022.

Methods

Department of Biochemistry & Molecular Genetics, School of Medicine, University of Colorado, 12801 E. 17(th) Avenue, Aurora, CO 80045, USA. Electronic address:

Published: October 2022

AI Article Synopsis

  • - The introduction of exact nuclear Overhauser enhancement (eNOE) in solution-state NMR spectroscopy allows for tighter distance restraints, improving structure calculations and resolving different macromolecular conformations.
  • - Between 2018 and 2022, significant technical advancements in eNOE were made, along with major applications that addressed various biological questions related to proteins, RNA, and DNA.
  • - This review highlights innovations in NMR methodologies, including non-uniform sampling, new pulse sequences, and enhanced data analysis techniques, as well as their impact on structural biology.

Article Abstract

The introduction of the exact nuclear Overhauser enhancement (eNOE) methodology to solution-state nuclear magnetic resonance (NMR) spectroscopy results in tighter distance restraints from NOEs than in convention analysis. These improved restraints allow for higher resolution in structure calculation and even the disentanglement of different conformations of macromolecules. While initial work primarily focused on technical development of the eNOE, structural studies aimed at the elucidation of spatial sampling in proteins and nucleic acids were published in parallel prior to 2018. The period of 2018-2022 saw a continued series of technical innovation, but also major applications addressing biological questions. Here, we review both aspects, covering topics from the implementation of non-uniform sampling of NOESY buildups, novel pulse sequences, adaption of the eNOE to solid-state NMR, advances in eNOE data analysis, and innovations in structural ensemble calculation, to applications to protein, RNA, and DNA structure elucidation.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9596134PMC
http://dx.doi.org/10.1016/j.ymeth.2022.08.006DOI Listing

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