AI Article Synopsis
- High-throughput proteomic profiling is increasingly used in human studies, but there is a lack of direct comparisons between antibody- and aptamer-based platforms.
- The study evaluated the performance of three profiling techniques—SomaScan1.3K, SomaScan5K, and Olink Explore—on 787 participants across various performance domains like precision and accuracy.
- Results indicated that the Olink platform showed better protein target specificity and phenotypic associations, while the Soma platforms excelled in measurement precision and broader analytical capabilities.
Article Abstract
High-throughput proteomic profiling using antibody or aptamer-based affinity reagents is used increasingly in human studies. However, direct analyses to address the relative strengths and weaknesses of these platforms are lacking. We assessed findings from the SomaScan1.3K ( = 1301 reagents), the SomaScan5K platform ( = 4979 reagents), and the Olink Explore ( = 1472 reagents) profiling techniques in 568 adults from the Jackson Heart Study and 219 participants in the HERITAGE Family Study across four performance domains: precision, accuracy, analytic breadth, and phenotypic associations leveraging detailed clinical phenotyping and genetic data. Across these studies, we show evidence supporting more reliable protein target specificity and a higher number of phenotypic associations for the Olink platform, while the Soma platforms benefit from greater measurement precision and analytic breadth across the proteome.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9390994 | PMC |
| http://dx.doi.org/10.1126/sciadv.abm5164 | DOI Listing |
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