Genome-wide analysis of genes in and functional characterization of in jasmonate-mediated immunity to .

, belonging to the Orchidaceae, is a precious Chinese herbal medicine. (P1) is a broad-spectrum fungal disease, which causes widespread loss in the near-wild cultivation of . Thus, resistance breeding of has become the key to solve this problem. The () gene family is closely related to plant resistance to external stresses, but the related research in is not deep enough yet. Phylogenetic analysis showed that 108 genes could be divided into 23 subgroups. Promoter cis-acting elements revealed that contain a large number of stress-related cis-acting elements. Transcriptome analysis of MeJA and P1 treatment manifested that exogenous MeJA can change the expression pattern of most genes, especially the IIIe subgroup, including inhibiting the expression of () and promoting the expression of (). Subcellular localization indicated that they were located in the nucleus. Furthermore, exogenous MeJA treatment significantly delayed disease time and reduced lesion size after infection with P1. -overexpression Arabidopsis lines showed significantly smaller lesions after being infected with P1 than the wild type, indicating that functions as an important positive regulator in defense against P1. Our findings shed more insights into the critical role of the family in plants and the resistance breeding of .