AI Article Synopsis
- Transmission electron microscopy (TEM) is an advanced imaging technique that allows researchers to view the detailed structure of cells and tissues at an atomic level.
- TEM provides critical insights into cellular processes and can complement functional analysis findings.
- The chapter offers practical protocols for preparing Drosophila tissues for TEM, including methods like classical chemical fixation and high-pressure freezing-freeze substitution (HPF-FS) to maintain cellular integrity.
Article Abstract
Transmission electron microscopy (TEM) is the method of choice to image the ultrastructure of cells or tissues. TEM allows the visualization of molecular complexes up to an atomic resolution. Thus, TEM data have led to important conclusions about cellular processes and supported findings obtained by functional analyses. In this chapter, we describe the preparation of Drosophila tissues for TEM and provide reliable step-by-step protocols for applying classical chemical fixation or high-pressure freezing-freeze substitution (HPF-FS) to preserve cellular structures.
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| http://dx.doi.org/10.1007/978-1-0716-2541-5_19 | DOI Listing |
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