AI Article Synopsis
- Cyclodextrin-based cryoprotectants were created, specifically α-TMCD, which can be produced on a large scale and demonstrated improved cell viabilities for various cell types.
- The use of α-TMCD resulted in a 103.2% increase in hucMSC cell numbers when observed under light microscopy.
- An α-TMCD-DMSO combination exhibited stronger cryoprotective effects through enhanced cell penetration and synergy between DMSO and α-TMCD, highlighting its potential in cryopreservation applications.
Article Abstract
Cyclodextrin-based cryoprotectants were developed. α-TMCD, which can be easily put into large-scale production, showed enhanced cell viabilities of 19.97 ± 0.78%, 13.93 ± 4.46% and 19.10 ± 0.95% against GES-1, hucMSCs and A549 cells. Moreover, the viable cells observed by light microscope imaging showed that the enhanced hucMSC cell number percentage of α-TMCD was 103.2%. An α-TMCD-DMSO-based CPA exhibited an enhanced cryoprotective effect by a mechanism of DMSO-enhanced cell penetrating effect and α-TMCD-DMSO synergistically enhanced IMA ability. α-TMCD exhibited potential for the discovery of macrocycle-molecule-based cryoprotectants.
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| http://dx.doi.org/10.1039/d2tb01083f | DOI Listing |
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