The replication crisis has gained traction in the scientific community over the past decade. Basic researchers have shown the reproducibility of key data from the lab is low and there is a clear need to improve lab protocol and technique.
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Genomic re-sequencing reveals mutational divergence across genetically engineered strains of model archaea.
mSystems
January 2025
Department of Biology, Duke University, Durham, North Carolina, USA.
Unlabelled: Archaeal molecular biology has been a topic of intense research in recent decades as their role in global ecosystems, nutrient cycles, and eukaryotic evolution comes to light. The hypersaline-adapted archaeal species and serve as important model organisms for understanding archaeal genomics, genetics, and biochemistry, in part because efficient tools enable genetic manipulation. As a result, the number of strains in circulation among the haloarchaeal research community has increased in recent decades.
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Research Laboratory Neuroelectronics and Memristive Nanomaterials (NEUROMENA Lab), Institute of Nanotechnologies, Electronics and Electronic Equipment Engineering, Southern Federal University, Taganrog 347922, Russia.
This paper presents the results of a study on the formation of nanostructures of electrochemical titanium oxide for neuromorphic applications. Three anodization synthesis techniques were considered to allow the formation of structures with different sizes and productivity: nanodot, lateral, and imprint. The mathematical model allowed us to calculate the processes of oxygen ion transfer to the reaction zone; the growth of the nanostructure due to the oxidation of the titanium film; and the formation of TiO, TiO, and TiO oxides in the volume of the growing nanostructure and the redistribution of oxygen vacancies and conduction channel.
View Article and Find Full Text PDFEvaluating the Potential of Microdroplet Flow in Two-Phase Biocatalysis: A Systematic Study.
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Institute of Microtechnology (IMT), Technische Universität Braunschweig, Alte Salzdahlumer Straße 203, DE-38124 Braunschweig, Germany.
Two-phase biocatalysis in batch reactions often suffers from inefficient mass transfer, inconsistent reaction conditions, and enzyme inactivation issues. Microfluidics offer uniform and controlled environments ensuring better reproducibility and enable efficient, parallel processing of many small-scale reactions, making biocatalysis more scalable. In particular, the use of microfluidic droplets can increase the interfacial area between the two phases and can therefore also increase reaction rates.
View Article and Find Full Text PDFFilter-assisted sample preparation for on-site detection using a bi-functional linker-based biosensor demonstrated with Escherichia coli O157:H7.
Food Chem
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Department of Agricultural Biotechnology, Seoul National University, 1 Gwanakro, Gwanakgu, Seoul, 08826, Republic of Korea; Center for Food and Bioconvergence, Seoul National University, 1 Gwanakro, Gwanakgu, Seoul, 08826, Republic of Korea; Research Institute for Agriculture and Life Sciences, Seoul National University, 1 Gwanakro, Gwanakgu, Seoul, 08826, Republic of Korea. Electronic address:
This study presents an advanced food detection platform that integrates filter-assisted sample preparation (FASP) with a bifunctional linker-based biosensor for on-site detection of Escherichia coli O157:H7 as a model case. FASP isolates bacteria from food samples through multi-filter preprocessing, significantly enhancing the specificity, sensitivity, and reproducibility of the subsequent biosensor analysis. This platform can detect E.
View Article and Find Full Text PDFThe mysterious case of missing lymphocytes: a cautionary tale of inter-institutional variability in outcomes of lung dissociation protocols.
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January 2025
Dept. of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA.
Rigor and reproducibility are vital to scientific advancement. It is unclear whether a protocol optimized for tissue dissociation in one institution performs well universally. Here, we share our brand-new lab's experience with inter-institutional variability that led to the discovery that a protocol optimized for murine lung dissociation at Boston University (BU) fails to reproduce similar CD4 T cell, CD8 T cell, and B cell outcomes at the University of Michigan at Ann Arbor (U-M).
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