RNA-sequencing analysis of bisphenol A biodegradation by white-rot fungus YK-624.

3 Biotech

Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou, 510006 China.

Published: September 2022

AI Article Synopsis

  • Bisphenol A (BPA) is a widely used endocrine-disrupting chemical that poses health risks, necessitating effective degradation methods.* -
  • The white-rot fungus YK-624 has shown capability to degrade BPA under various conditions, but the specific enzymes involved remained unclear until this study.* -
  • RNA sequencing revealed that lignin peroxidase and cytochrome P450 play key roles in BPA degradation, with findings validated by quantitative real-time PCR.*

Article Abstract

Unlabelled: Bisphenol A (BPA) is a representative example of an endocrine-disrupting chemical. It is one of the most produced chemical substances in the world, but it causes harmful effects in organisms, such that the effective degradation of BPA is critical. The white-rot fungus YK-624 has been shown to effectively degrade BPA under ligninolytic and non-ligninolytic conditions. However, it is still unclear what kinds of enzymes are involved in BPA degradation. To explore the mechanism of BPA degradation, the present study analysed the functional genes of YK-624 using RNA-sequencing (RNA-Seq). Oxidation-reduction process and metabolic pathway were enriched under ligninolytic and non-ligninolytic conditions by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. It is suggested that BPA might be used as a carbon source by YK-624. Lignin peroxidase and cytochrome P450 were detected in upregulated differentially expressed genes (DEGs). The lignin-degrading enzyme lignin peroxidase and the intracellular cytochrome P450 system were involved in BPA degradation by YK-624, respectively. Furthermore, quantitative real-time PCR (qPCR) was used to validate the reliability of the RNA-Seq results.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03298-w.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375798PMC
http://dx.doi.org/10.1007/s13205-022-03298-wDOI Listing

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