AI Article Synopsis
- Drug toxicity, particularly from acetaminophen (APAP), can lead to serious liver and kidney damage due to the excessive production of hypochlorous acid (HClO) in these organs.
- Research developed a chemodosimeter, NRH-O, which specifically responds to HClO, allowing for the monitoring of its levels in living cells and highlighting increases caused by APAP.
- NRH-O also serves as a diagnostic tool for liver and kidney injuries, utilizing fluorescence ratio imaging to visualize HClO levels in inflammatory mouse tissues.
Article Abstract
Liver and kidney injury caused by drug toxicity is a serious threat to human health. Acetaminophenol (APAP), as a common antipyretic and analgesic drug, inevitably causes injury. When it is overused, hypochlorous acid (HClO) is excessively generated due to metabolic abnormalities, resulting in the accumulation of HClO in the mitochondria of liver and kidney tissues and causing damage. In this study, we designed a series of HClO responsive ratiometric chemdosimeter NRH-X (NRH-O, NRH-S, and NRH-C) to evaluate liver and kidney injury, and found that NRH-O has a specific sensitive response to HClO. NRH-O can not only monitor the variations of endogenous HClO content of living cells by fluorescence ratio changes in the mitochondria but also detect the upregulation of HClO induced by APAP. In addition, NRH-O can also be used for anatomic diagnosis of liver and kidney injury by fluorescence ratio imaging of HClO in the tissues of inflammatory mice.
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| http://dx.doi.org/10.1021/acs.analchem.2c02431 | DOI Listing |
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