Two aerobic, Gram-stain-negative, motile, mesophilic, rod-shaped and catalase-positive bacterial strains designated AF9R3 and GN2-R2 were isolated from flowers collected in the Republic of Korea. Strain AF9R3 grew at 4-33 °C, pH 4.0-9.0 and with 0-1 % NaCl (w/v), and strain GN2-R2 grew at 10-33 °C, pH 4.0-9.0 and with 0-1 % NaCl (w/v). Phylogenetic analysis on the basis of 16S rRNA gene sequences indicated that strains AF9R3 and GN2-R2 belonged to the genera and , respectively, showing high sequence similarity to CY42W (99.4 %) and 6 NM-7 (98.0 %), respectively. Both strains contained summed feature 3 (C 7 and/or C 6) and C as the major fatty acids, and ubiquinone Q-8 as the predominant quinone. Strain AF9R3 had diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and strain GN2-R2 comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as the major polar lipids. Orthologous average nucleotide identity and digital DNA-DNA hybridization values of strain AF9R3 to its closest relative CY42W were 92.6 and 56.5 %, and those of strain GN2-R2 to its closest relative 6 NM-7 were 81.4 and 24.8 %. Based on genotypic and phenotypic data, strains AF9R3 and GN2-R2 are considered to represent novel species of the genus and , respectively, for which the names sp. nov. (type strain AF9R3=KACC 21258=NBRC 114510) and sp. nov. (type strain GN2-R2=KACC 21261=NBRC 114511) have been proposed.
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http://dx.doi.org/10.1099/ijsem.0.005487 | DOI Listing |
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