Pleiotropic Effects of Ticagrelor: Influence on Gene and Protein Expression in HUVEC and HepG2, and Bacterial Survival.

Background: Antiplatelet drugs, such as ticagrelor, which target platelet P2Y receptors, are used for prevention of ischemic heart disease. Ticagrelor is also known to have pleiotropic effects of unknown mechanisms. Ticagrelor could influence the expression of molecules involved in resolution of inflammation. This study aimed to investigate if ticagrelor could change the expression of and its encoded protein concentration and, additionally, to determine ticagrelor possible antibacterial activity against gram-negative bacteria.

Methods: expression was determined in HUVEC and HepG2 cell lines by qPCR. CYP4F2 protein concentration was determined by ELISA. Antibiotic susceptibility testing was performed using a disc diffusion method.

Results: Ticagrelor was observed to reduce the expression of in HUVEC and HepG2 cell lines. It also reduced CYP4F2 protein levels in HUVEC cells. Ticagrelor had no bactericidal activity against gram-negative third generation cephalosporin resistant .

Conclusion: Ticagrelor reduced CYP4F2 protein concentration in HUVEC, and expression in HUVEC and HepG2 cells, but had no effect on third-generation cephalosporin-resistant strains.