7α,25-dihydroxycholesterol (7α,25-DHC) is an oxysterol synthesized from 25-hydroxycholesterol by cytochrome P450 family 7 subfamily B member 1 (CYP7B1) and is a monooxygenase (oxysterol-7α-hydroxylase) expressed under inflammatory conditions in various cell types. In this study, we verified that 7α,25-DHC-induced oxiapoptophagy is mediated by apoptosis, oxidative stress, and autophagy in L929 mouse fibroblasts. MTT assays and live/dead cell staining revealed that cytotoxicity was increased by 7α,25-DHC in L929 cells. Consequentially, cells with condensed chromatin and altered morphology were enhanced in L929 cells incubated with 7α,25-DHC for 48 h. Furthermore, apoptotic population was increased by 7α,25-DHC exposure through the cascade activation of caspase-9, caspase-3, and poly (ADP-ribose) polymerase in the intrinsic pathway of apoptosis in these cells. 7α,25-DHC upregulated reactive oxygen species (ROS) in L929 cells. Expression of autophagy biomarkers, including beclin-1 and LC3, was significantly increased by 7α,25-DHC treatment in L929 cells. 7α,25-DHC inhibits the phosphorylation of Akt associated with autophagy and increases p53 expression in L929 cells. In addition, inhibition of G-protein-coupled receptor 183 (GPR183), a receptor of 7α,25-DHC, using GPR183 specific antagonist NIBR189 suppressed 7α,25-DHC-induced apoptosis, ROS production, and autophagy in L929 cells. Collectively, GPR183 regulates 7α,25-DHC-induced oxiapoptophagy in L929 cells.
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http://dx.doi.org/10.3390/molecules27154798 | DOI Listing |
Microb Pathog
December 2024
State University of Ceará, Northeast Network of Biotechnology Program (RENORBIO), Campus Itaperi, Fortaleza, Brazil; Course of Chemistry, State University of Vale Acaraú, Sobral, Ceará, Brazil; Postgraduate in Natural Sciences, Sciences and Technology Center, State University of Ceará, Fortaleza, CE, Brazil. Electronic address:
The study investigates the synthesis, characterization, and antibacterial activity of an ibuprofen-derived hydrazide (HIDZ). It was synthesized and characterized using NMR spectroscopy, DFT Calculations, and ADMET studies. Furthermore, HIDZ cytotoxicity on L929 cells was evaluated using the MTT reduction assay.
View Article and Find Full Text PDFFront Oncol
December 2024
Department of Occupational Medicine, Tainan Municipal Hospital (managed by ShowChwan Medical Care Corporation), Tainan, Taiwan.
Introduction: Cancer has emerged as one of the leading causes of fatality all over the world. Phytoconstituents are being studied for their synergistic effects, which include disease prevention by altering molecular pathways and immunomodulation without side effects. The present experiment aims to explore the cancer preventive activities of Linn leaves extract in skin cancer cell lines (A431) and colon cancer cell lines (COLO 320DM)).
View Article and Find Full Text PDFPolymers (Basel)
November 2024
Department of Biomaterials and Cosmetic Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7, 87-100 Torun, Poland.
In this work, new materials based on the blends of polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), chitosan (CS), and polydopamine (PDA) have been prepared. Fourier Transform Infrared Spectra have been conducted to verify the presence of individual components in the composite materials. EDX elemental analysis showed a clear view of the element's presence in the composite materials, with the maximum values for carbon and oxygen.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Department of Electrical Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran.
Biological macromolecules such as polysaccharides and proteins, due to their excellent biocompatibility and biodegradability, are ideal for promoting Skin Tissue Engineering (STE) both in vitro and in vivo. In this study, a core-shell electrospun scaffold was fabricated using the coaxial electrospinning method, with Polyurethane (PU) forming the shell and a mixture of Starch (ST), Propolis Extract (PE), and Hyaluronic Acid (HA) forming the core. The scaffold's morphology was characterized by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM), confirming the successful formation of a well-defined core-shell structure.
View Article and Find Full Text PDFBr J Pharmacol
December 2024
School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
Background And Purpose: Stimulator of interferon response cGAMP interactor 1 (STING), a central hub protein of cyclic GMP-AMP synthase (cGAS)-STING signalling pathway, has a crucial role in regulating type I interferons (IFNs) production and response. Recent studies indicate that excessive activation of STING is strongly associated with autoimmune diseases, including systemic lupus erythematosus (SLE). Searching immunomodulators that negatively regulate STING might greatly contribute to the suppression of autoimmunity.
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