AI Article Synopsis
- - The study investigates how chloroplast reactive oxygen species and salicylic acid (SA) signaling relate to the hypersensitive response (HR) in plants, particularly in resisting potato virus Y (PVY) in a resistant potato genotype.
- - Researchers found that while chloroplast redox changes near cell death zones signal resistance, fewer oxidized chloroplasts appeared in SA-deficient plants, suggesting a crucial role for SA in resistance signaling.
- - The analysis also revealed that stromules (extensions of chloroplasts) are induced by cell death and PVY multiplication, but this response is significantly reduced in plants lacking SA.
Article Abstract
Hypersensitive response (HR)-conferred resistance is associated with induction of programmed cell death and pathogen spread restriction in its proximity. The exact role of chloroplastic reactive oxygen species and its link with salicylic acid (SA) signaling in HR remain unexplained. To unravel this, we performed a detailed spatiotemporal analysis of chloroplast redox response in palisade mesophyll and upper epidermis to potato virus Y (PVY) infection in a resistant potato genotype and its transgenic counterpart with impaired SA accumulation and compromised resistance. Besides the cells close to the cell death zone, we detected individual cells with oxidized chloroplasts further from the cell death zone. These are rare in SA-deficient plants, suggesting their role in signaling for resistance. We confirmed that chloroplast redox changes play important roles in signaling for resistance, as blocking chloroplast redox changes affected spatial responses at the transcriptional level. Through spatiotemporal study of stromule induction after PVY infection, we show that stromules are induced by cell death and also as a response to PVY multiplication at the front of infection. Overall induction of stromules is attenuated in SA-deficient plants.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9875368 | PMC |
| http://dx.doi.org/10.1111/nph.18425 | DOI Listing |
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