AI Article Synopsis
- An essential gene is crucial for a cell's survival, but determining which genes are essential isn’t always straightforward, especially in yeast.
- Research shows there are two types of essential genes: core essential genes that are always necessary for life and conditional essential genes that can become important depending on the genetic makeup and environmental factors.
- This review focuses on the latest findings about gene essentiality in yeast, highlighting how core essential genes differ from those that depend on specific conditions or contexts.
Article Abstract
An essential gene encodes for a cellular function that is required for viability. Although viability is a straightforward phenotype to analyze in yeast, defining a gene as essential is not always trivial. Gene essentiality has generally been studied in specific laboratory strains and under standard growth conditions, however, essentiality can vary across species, strains, and environments. Recent systematic studies of gene essentiality revealed that two sets of essential genes exist: core essential genes that are always required for viability and conditional essential genes that vary in essentiality in different genetic and environmental contexts. Here, we review recent advances made in the systematic analysis of gene essentiality in yeast and discuss the properties that distinguish core from context-dependent essential genes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.gde.2022.101963 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Machine learning methods for predicting essential metabolic genes from Plasmodium falciparum genome-scale metabolic network.
PLoS One
December 2024
Department of Computer and Information Sciences, Covenant University, Ota, Ogun State, Nigeria.
Essential genes are those whose presence is vital for a cell's survival and growth. Detecting these genes in disease-causing organisms is critical for various biological studies, including understanding microbe metabolism, engineering genetically modified microorganisms, and identifying targets for treatment. When essential genes are expressed, they give rise to essential proteins.
View Article and Find Full Text PDFGO-CRISPR: A highly controlled workflow to discover gene essentiality in loss-of-function screens.
PLoS One
December 2024
London Health Sciences Centre Research Institute, London Regional Cancer Program, London, ON, Canada.
Genome-wide CRISPR screens are an effective discovery tool for genes that underlie diverse cellular mechanisms that can be scored through cell fitness. Loss-of-function screens are particularly challenging compared to gain-of-function because of the limited dynamic range of decreased sgRNA sequence detection. Here we describe Guide-Only control CRISPR (GO-CRISPR), an improved loss-of-function screening workflow, and its companion software package, Toolset for the Ranked Analysis of GO-CRISPR Screens (TRACS).
View Article and Find Full Text PDFEvaluation of Spliceosome Protein SmD2 as a Potential Target for Cancer Therapy.
Int J Mol Sci
December 2024
Amsterdam UMC location Vrije Universiteit Amsterdam, Medical Oncology, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands.
The core spliceosome Sm proteins are gaining attention as potential targets for cancer treatment. Here, we evaluate this, with focus on SmD2. A pan-cancer analysis including 26 solid tumor types revealed that the SmD2-encoding gene was overexpressed in almost all cancers.
View Article and Find Full Text PDFAn RNAi screen of Rab GTPase genes in reveals that somatic cells of the reproductive system depend on for morphogenesis but not stem cell niche maintenance.
bioRxiv
December 2024
Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599.
Membrane trafficking is a crucial function of all cells and is regulated at multiple levels from vesicle formation, packaging, and localization to fusion, exocytosis, and endocytosis. Rab GTPase proteins are core regulators of eukaryotic membrane trafficking, but developmental roles of specific Rab GTPases are less well characterized, potentially because of their essentiality for basic cellular function. gonad development entails the coordination of cell growth, proliferation, and migration-processes in which membrane trafficking is known to be required.
View Article and Find Full Text PDFImpact of Vitamin A Deficiency on Iron Metabolism and Anemia: A Historical Perspective and Research Advances.
Nutr Rev
December 2024
Department of Food Science and Human Nutrition, University of Illinois Urbana-Champaign, Urbana, IL 61801, United States.
Vitamin A deficiency (VAD) and iron deficiency anemia coexist around the world, particularly in children and women of reproductive age in low- and middle-income countries. Within this scenario, there is a known interaction between vitamin A and iron, and it has been postulated that lack of vitamin A impairs iron metabolism, leading to vitamin A deficiency anemia (VADA). Current animal, epidemiological, and clinical studies support this notion.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!