Evaluation of the toxicology, anti-lipase, and antioxidant effects of in rats fed with a high fat-fructose diet.

Context: Skeels (Myrtaceae) exhibits many biological activities.

Objective: This study analyzes for the first time, the toxicity, obesogenic, and antioxidant effects of in rats fed with a high fat-fructose diet (HFFD).

Materials And Methods: Four studies using male Wistar rats were conducted: (a) 7 groups ( = 3): control (corn oil) and ethanol extract of leaf (single oral dose at 100-4000 mg/kg) for acute toxicity; (b) 2 groups ( = 8): control (corn oil) and (1000 mg/kg/day) for 28 days for subacute toxicity; (c) 3 groups ( = 4) with single oral dose of lipid emulsion: control (lipid emulsion), and orlistat (250 and 50 mg/kg, respectively) for lipid absorption; (d) 4 groups ( = 6): control (normal diet) and 3 groups fed with HFFD: HFFD only, and simvastatin (oral dose 250 and 3 mg/kg, respectively) for 13 weeks. Antioxidant enzymes and biomarkers were evaluated and inhibition of pancreatic lipase was determined .

Results: Toxicological studies of showed no differences in biochemical parameters and lethal dose (LD) was higher than 4000 mg/kg. inhibited pancreatic lipase activity, with IC of 392.00 µg/mL, and decreased lipid absorption by 70%. Additionally, it reduced the body weight 22%, restored the activities of antioxidant enzymes, and reduced the biomarkers of oxidative stress.

Conclusions: showed an effect against oxidative stress by reducing biomarkers and induced antioxidant system, without toxic effects.