Structure of fungal communities in sequencing batch reactors operated at different salinities for the selection of triacylglyceride-producers from a fish-canning lipid-rich waste stream.

N Biotechnol

Microbiology Department, Faculty of Pharmacy, University of Granada, 18001 Granada, Andalucía, Spain; Microbiology and Environmental technology section, Microbiology Department, Faculty of Pharmacy, University of Granada, 18011 Granada, Andalucía, Spain.

Published: November 2022

AI Article Synopsis

  • The study investigated the potential of oleaginous fungi in accumulating triacylglycerides (TAG) from lipid-rich waste generated by the fish-canning industry for sustainable biodiesel production.* -
  • Researchers operated two sequencing batch reactors (SBRs) under different salt concentrations to enrich fungal mixed microbial cultures (MMCs) capable of high TAG storage while analyzing the fungal community using advanced molecular techniques.* -
  • Results showed an increase in fungal diversity and abundance, with specific TAG-accumulating genera identified, but the connection between these genera and enhanced TAG accumulation was not significantly established.*

Article Abstract

Oleaginous fungi natively accumulate large amounts of triacylglycerides (TAG), widely used as precursors for sustainable biodiesel production. However, little attention has been paid to the diversity and roles of fungal mixed microbial cultures (MMCs) in sequencing batch reactors (SBR). In this study, a lipid-rich stream produced in the fish-canning industry was used as a substrate in two laboratory-scale SBRs operated under the feast/famine (F/F) regime to enrich microorganisms with high TAG-storage ability, under two different concentrations of NaCl (SBR-N: 0.5 g/L; SBR-S: 10 g/L). The size of the fungal community in the enriched activated sludge (EAS) was analyzed using 18S rRNA-based qPCR, and the fungal community structure was determined by Illumina sequencing. The different selective pressures (feeding strategy and control of pH) implemented in the enrichment SBRs throughout operation increased the abundance of total fungi. In general, there was an enrichment of genera previously identified as TAG-accumulating fungi (Apiotrichum, Candida, Cutaneotrichosporon, Geotrichum, Haglerozyma, Metarhizium, Mortierella, Saccharomycopsis, and Yarrowia) in both SBRs. However, the observed increase of their relative abundances throughout operation was not significantly linked to a higher TAG accumulation.

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http://dx.doi.org/10.1016/j.nbt.2022.08.001DOI Listing

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