Objective: To define the potential of polycaprolactone (PCL) scaffold for cementoblast delivery.
Background: Dental cementum is critical for tooth attachment and position, and its regenerative capabilities remain unpredictable.
Methods: PCL scaffolds were manufactured by the electrospinning technique at 10% and 20% (w/v) and seeded with cementoblasts (OCCM-30). Scaffolds were characterized for their morphology and biological performance by scanning electron microscopy (SEM), confocal and conventional histology, cytocompatibility (PrestoBlue assay), gene expression (type I collagen - Col1; bone sialoprotein - Bsp; runt-related transcription factor 2 - Runx-2; alkaline phosphatase - Alpl; osteopontin - Opn; osteocalcin - Ocn, osterix - Osx), and the potential to induce extracellular matrix deposition and mineralization in vitro.
Results: Overall, data analysis showed that PCL scaffolds allowed cell adhesion and proliferation, modulated the expression of key markers of cementoblasts, and led to enhanced extracellular matrix deposition and calcium deposition as compared to the control group.
Conclusion: Altogether, our findings allow concluding that PCL scaffolds are a viable tool to culture OCCM-30 cells, leading to an increased potential to promote mineralization in vitro. Further studies should be designed in order to define the clinical relevance of cementoblast-loaded PCL scaffolds to promote new cementum formation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/jre.13041 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of the Mechanical and Biological Properties of Polycaprolactone Scaffolds Produced by a Material Extrusion 3D Printer or 3D Pen: A Novel Bone Repair Strategy.
J Biomed Mater Res B Appl Biomater
January 2025
Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul, Republic of Korea.
Addressing the high cost and long cycle associated with the multistep digital restoration process involving 3D printing technology, we proposed the 3D pen as an innovative strategy for rapid bone repair. Capitalizing on the low melting point characteristic of polycaprolactone (PCL), we introduced, for the first time, the novel concept of directly constructing scaffolds at bone defect sites using 3D pens. In this in vitro study, we meticulously evaluated both the mechanical and biological properties of 3D pen-printed PCL scaffolds with six distinct textures: unidirectional (UNI) (0°, 45°, 90°), bidirectional (BID) (-45°/45°, 0°/90°), and concentric (CON).
View Article and Find Full Text PDFEvaluation of the bone regenerative effect of glycogen synthase kinase 3 antagonist Tideglusib carried by different scaffolds on rat calvarial defects.
Int J Biol Macromol
December 2024
Department of Periodontology, Faculty of Dentistry, Marmara University, Istanbul 34854, Turkiye. Electronic address:
The aim was to explore the efficiency of Tideglusib in bone tissue healing by carrying it with different scaffolds on rat calvarial lesions. Twentyfour male Dawley rats were utilized. Two bone defects of 5 mm in diameter were formed (n = 8).
View Article and Find Full Text PDFBiomaterials for Corneal Regeneration.
Adv Sci (Weinh)
December 2024
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
Corneal blindness is a significant reason for visual impairment globally. Researchers have been investigating several methods for corneal regeneration in order to cure these patients. Biomaterials are favored due to their biocompatibility and capacity to promote cell adhesion.
View Article and Find Full Text PDF3D-printed TiC/polycaprolactone composite scaffold with a DOPA-SDF1 surface modified for bone repair.
Colloids Surf B Biointerfaces
December 2024
Department of Orthopaedic Surgery, Orthopaedic Center, The First Hospital of Jilin University, Changchun 130021, China. Electronic address:
Large bone defects are a major clinical challenge in bone reconstructive surgery. 3D printing is a powerful technology that enables the manufacture of custom tissue-engineered scaffolds for bone regeneration. Electrical stimulation (ES) is a treatment method for external bone defects that compensates for damaged internal electrical signals and stimulates cell proliferation and differentiation.
View Article and Find Full Text PDFIntegrating electrospun aligned fiber scaffolds with bovine serum albumin-basic fibroblast growth factor nanoparticles to promote tendon regeneration.
J Nanobiotechnology
December 2024
State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China.
Background: Electrospun nanofiber scaffolds have been widely used in tissue engineering because they can mimic extracellular matrix-like structures and offer advantages including high porosity, large specific surface area, and customizable structure. In this study, we prepared scaffolds composed of aligned and random electrospun polycaprolactone (PCL) nanofibers capable of delivering basic fibroblast growth factor (bFGF) in a sustained manner for repairing damaged tendons.
Results: Aligned and random PCL fiber scaffolds containing bFGF-loaded bovine serum albumin (BSA) nanoparticles (BSA-bFGF NPs, diameter 146 ± 32 nm) were fabricated, respectively.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!