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Proteotypic Differences of Follicular-Patterned Thyroid Neoplasms. | LitMetric

AI Article Synopsis

  • - The study investigated the molecular differences between follicular thyroid tumors, specifically follicular adenoma (FA), follicular carcinoma (FTC), and follicular variant of papillary thyroid carcinoma (FvPTC), using proteomic analysis and advanced mass spectrometry techniques.
  • - A total of 4,107 proteins were analyzed from 52 tumor specimens, revealing significant differences in protein expression: 287 proteins differed between FTC and FA, 303 between FvPTC and FA, and 23 between FTC and FvPTC.
  • - The findings highlighted ANXA1 as a key protein biomarker that can effectively distinguish FvPTC from both FA and FTC, aiding in the diagnosis of these challenging tumors. *

Article Abstract

The diagnosis of follicular-patterned thyroid tumors such as follicular thyroid adenoma (FA), follicular thyroid carcinoma (FTC), and follicular variant of papillary thyroid carcinoma (FvPTC) remains challenging. This study aimed to explore the molecular differences among these three thyroid tumors by proteomic analysis. A pressure cycling technology (PCT)-data-independent acquisition (DIA) mass spectrometry workflow was employed to investigate protein alterations in 52 formalin-fixed paraffin-embedded (FFPE) specimens: 18 FA, 15 FTC, and 19 FvPTC specimens. Immunohistochemical (IHC) analysis of 101 FA, 67 FTC, and 65 FvPTC specimens and parallel reaction monitoring (PRM) analysis of 20 FA, 20 FTC, and 20 FvPTC specimens were performed to validate protein biomarkers. A total of 4107 proteins were quantified from 52 specimens. Pairwise comparisons identified 287 differentially regulated proteins between FTC and FA, and 303 between FvPTC and FA and 88 proteins were co-dysregulated in the two comparisons. However, only 23 discriminatory proteins between FTC and FvPTC were detected. Additionally, the quantitative results for ANXA1 expression based on IHC staining and PRM-MS quantification were consistent with the proteomic results, showing that ANXA1 can be used to distinguish FvPTC from FA and FTC. The differentially regulated proteins found in this study can differentiate FA from FvPTC. In addition, ANXA1 is a promising biomarker for differentiating FvPTC from the other thyroid tumors.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340356PMC
http://dx.doi.org/10.3389/fendo.2022.854611DOI Listing

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