AI Article Synopsis
- PLA (3-D-phenyllactic acid) is a beneficial antimicrobial and immune regulatory compound found in honey and fermented foods, and S. inulinus is identified as an effective producer of this compound.
- Researchers cloned and expressed three genes encoding D-lactate dehydrogenase (D-LDH) in E. coli, resulting in the production of 47 mM D-PLA with an 88% conversion yield.
- D-LDH1 was the most effective enzyme for converting phenylpyruvate to PLA, with key findings indicating that the Ile307 residue plays a crucial role in facilitating this efficient conversion process.
Article Abstract
PLA (3-D-phenyllactic acid) is an ideal antimicrobial and immune regulatory compound present in honey and fermented foods. Sporolactobacillus inulinus is regarded as a potent D-PLA producer that reduces phenylpyruvate (PPA) with D-lactate dehydrogenases. In this study, PLA was produced by whole-cell bioconversion of S. inulinus ATCC 15538. Three genes encoding D-lactate dehydrogenase (d-ldh1, d-ldh2, and d-ldh3) were cloned and expressed in Escherichia coli BL21 (DE3), and their biochemical and structural properties were characterized. Consequently, a high concentration of pure D-PLA (47 mM) was produced with a high conversion yield of 88%. Among the three enzymes, D-LDH1 was responsible for the efficient conversion of PPA to PLA with kinetic parameters of Km (0.36 mM), k (481.10 s ), and k /Km (1336.39 mM s ). In silico structural analysis and site-directed mutagenesis revealed that the Ile307 in D-LDH1 is a key residue for excellent PPA reduction with low steric hindrance at the substrate entrance. This study highlights that S. inulinus ATCC 15538 is an excellent PLA producer, equipped with a highly specific and efficient D-LDH1 enzyme.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618312 | PMC |
| http://dx.doi.org/10.1111/1751-7915.14125 | DOI Listing |
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