Diclofenac (DCF) is a pharmaceutically active contaminant frequently found in aquatic ecosystems. The transformation pathways and microbiology involved in the biodegradation of DCF, particularly under anoxic conditions, remain poorly understood. Here, we demonstrated microbially mediated reductive dechlorination of DCF in anaerobic enrichment culture derived from contaminated river sediment. Over 90% of the initial 76.7 ± 3.6 μM DCF was dechlorinated at a maximum rate of 1.8 ± 0.3 μM day during a 160 days' incubation. Mass spectrometric analysis confirmed that 2-(2-((2-chlorophenyl)amino)phenyl)acetic acid (2-CPA) and 2-anilinophenylacetic acid (2-APA) were formed as the monochlorinated and nonchlorinated DCF transformation products, respectively. A survey of microbial composition and Sanger sequencing revealed the enrichment and dominance of a new population, designated as sp. strain DCF, in the DCF-dechlorinating community. Following the stoichiometric conversion of DCF to 2-CPA (76.0 ± 2.1 μM) and 2-APA (3.7 ± 0.8 μM), strain DCF cell densities increased by 24.4 ± 4.4-fold with a growth yield of 9.0 ± 0.1 × 10 cells per μmol chloride released. Our findings expand the metabolic capability in the genus and highlight the relevant roles of organohalide-respiring bacteria for the natural attenuation of halogenated contaminants of emerging concerns (e.g., DCF).
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http://dx.doi.org/10.1021/acs.est.1c08824 | DOI Listing |
Toxicon
January 2025
Laboratory of Experimental Pathophysiology, Health Sciences, Universidade do Extremo Sul Catarinense. Universitário, 88806-000. Criciúma. Santa Catarina, Brazil. Electronic address:
Tityus serrulatus accident promote vast symptomatology related to toxins of the venom, which leads to a massive release of neurotransmitters, notably dopamine, affecting behavior and neurochemistry. The recommended treatment for envenomation is the antiscorpionic serum (SAEsc) administration. Related to this complexity of the Tityus serrulatus envenomation, this study aimed to assess organism responses to the venom, its impact on behavior, oxidative stress, neurochemistry, and genetic impacts, as well as the efficacy of SAEsc, especially concerning dopamine levels and genetic interactions.
View Article and Find Full Text PDFEnviron Toxicol Pharmacol
January 2025
Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat 390002, India. Electronic address:
Pyrethroids pose a great concern to the declining population of dung beetles and the sustainability of ecosystem services. This study aimed to investigate the toxic effects of deltamethrin on Digitonthophagus gazella. First, the LC value (0.
View Article and Find Full Text PDFJ Environ Manage
January 2025
Sanitary Engineering Laboratory, Department of Water Resources and Environmental Engineering, School of Civil Engineering, National Technical University of Athens, 9 Iroon Polytechniou St., Zographou Campus, 15773, Athens, Greece.
Vertical subsurface flow constructed wetlands (VSSF CWs) were employed to investigate the use of biochar that could be produced with local agricultural biomass through pyrolysis, recycled glass from local recycling companies and gel beads with decreased packing volume and shipping cost as substrate alternatives to sand. The materials were assessed in terms of granulometry, porosity, adsorption capacity and hydraulic conductivity and were used for the treatment of an upflow anaerobic sludge blanket (UASB) reactor, treating domestic wastewater, effluent. Granulometry was a major factor impacting TSS removal that ranged from 81% ± 10% to 97% ± 2%.
View Article and Find Full Text PDFJ Intell
December 2024
Department of Educational Studies in Psychology, Research Methodology, and Counseling, College of Education, The University of Alabama, Tuscaloosa, AL 35487, USA.
Mental rotation is an important aspect of spatial ability. While the importance of measuring mental rotation has been explored, disputes still exist within the literature surrounding sources of item difficulty in mental rotation tests (MRTs). Furthermore, gender differences in MRT performance are often seen but not fully understood.
View Article and Find Full Text PDFCarbohydr Polym
March 2025
State Key Laboratory for Pollution Control, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
The conventional hydrothermal synthesis and inherent hysteresis behavior limited the application of MOFs owing to the low kinetic efficiency in dynamic molecular adsorption. Herein, we developed an in-situ nucleation strategy for the preparation of MIL-100-Fe and immobilized it with hierarchy porous scaffold of TEMPO oxidized cellulose nanofiber (TCNF) sponge in the absence of additional organic solvent during fabrication under ambient conditions. The newly recognized mechanisms of gradient molecular transfer were proposed to illustrate the comprehensive DCF adsorption process from solution to micropores of MIL-100-Fe at molecule level triggered by the stray capacitance, varied Laplace pressure, size exclusion and cellulosic labyrinth.
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