Evaluation of the RESIST-4 O.K.N.V. K-SeT for detection of carbapenemases in Gram-negative bacilli.

Enterobacterales as opportunistic pathogens are commonly associated with nosocomial infections. With increasing frequency, Gram-negative bacilli, especially Klebsiella pneumoniae strains, are mul- tidrug-resistant or pandrug-resistant. Carbapenems were used as the drugs of choice for the treat- ment of infections caused by multidrug-resistant Gram-negative bacilli. The aim of this study was to assess the usefulness of the RESIST-4 O.K.N.V. K-SeT for the rapid detection and identification of the most important carbapenemases (OXA-48, KPC, NDM, VIM) in Enterobacterales bacilli. The study involved the isolates of 97 Enterobacterales strains. The ability to produce carbapenemases was determined by the immunochromatographic RESIST-4 O.K.N.V. K-SeT test. This test detected carbapenemases OXA-48, KPC, NDM, and VIM. For the RESIST-4 O.K.N.V. K-SeT test, a positive result was obtained for 93 strains (95.9%). Four strains negative in the RESIST-4 O.K.N.V. K-SeT were positive in the Eazyplex®SuperBugCRE and PCR. These strains produce VIM enzymes. RE- SIST-4 O.K.N.V. K-SeT test is rapid, simple to perform and can be used for fast detection of the most important carbapenemases (OXA-48, KPC, NDM, VIM) among Gram-negative bacilli.