Involvement of MdWRKY40 in the defense of mycorrhizal apple against fusarium solani.

BMC Plant Biol

State Key Laboratory of Crop Biology / College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.

Published: August 2022

Background: Apple (Malus domestica Borkh.) is an important economic crop. The pathological effects of Fusarium solani, a species complex of soilborne pathogens, on the root systems of apple plants was unknown. It was unclear how mycorrhizal apple seedlings resist infection by F. solani. The transcriptional profiles of mycorrhizal and non-mycorrhizal plants infected by F. solani were compared using RNA-Seq.

Results: Infection with F. solani significantly reduced the dry weight of apple roots, and the roots of mycorrhizal apple plants were less damaged when the plants were infected with F. solani. They also had enhanced activity of antioxidant enzymes and a reduction in the oxidation of membrane lipids. A total of 1839 differentially expressed genes (DEGs) were obtained after mycorrhizal and non-mycorrhizal apple plants were infected with F. solani. A gene ontogeny (GO) analysis showed that most of the DEGs were involved in the binding of ADP and calcium ions. In addition, based on a MapMan analysis, a large number of DEGs were found to be involved in the response of mycorrhizal plants to stress. Among them, the overexpressed transcription factor MdWRKY40 significantly improved the resistance of the apple 'Orin' callus to F. solani and the expression of the resistance gene MdGLU by binding the promoter of MdGLU.

Conclusion: This paper outlines how the inoculation of apple seedlings roots by arbuscular mycorrhizal fungi responded to infection with F. solani at the transcriptional level. In addition, MdWRKY40 played an important role in the resistance of mycorrhizal apple seedlings to infection with F. solani.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344649PMC
http://dx.doi.org/10.1186/s12870-022-03753-zDOI Listing

Publication Analysis

Top Keywords

mycorrhizal apple
16
infection solani
16
apple plants
12
apple seedlings
12
plants infected
12
infected solani
12
apple
10
solani
10
mycorrhizal
8
fusarium solani
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!